VLN2 Regulates Plant Architecture by Affecting Microfilament Dynamics and Polar Auxin Transport in Rice

doi:10.1105/tpc.15.00581

	VLN2 Regulates Plant Architecture by Affecting Microfilament Dynamics and Polar Auxin Transport in Rice
	Wu, Shengyang 1,2; Xie, Yurong 3; Zhang, Junjie 1,2; Ren, Yulong 2; Zhang, Xin 2; Wang, Jiulin 2; Guo, Xiuping 2; Wu, Fuqing 2; Sheng, Peike 2; Wang, Juan 4
刊名	PLANT CELL
	2015
卷号	27 期号:10 页码:2829-2845
ISSN号	1040-4651
DOI	10.1105/tpc.15.00581
通讯作者	Wu, Shengyang
英文摘要	As a fundamental and dynamic cytoskeleton network, microfilaments (MFs) are regulated by diverse actin binding proteins (ABPs). Villins are one type of ABPs belonging to the villin/gelsolin superfamily, and their function is poorly understood in monocotyledonous plants. Here, we report the isolation and characterization of a rice (Oryza sativa) mutant defective in VILLIN2 (VLN2), which exhibits malformed organs, including twisted roots and shoots at the seedling stage. Cellular examination revealed that the twisted phenotype of the vln2 mutant is mainly caused by asymmetrical expansion of cells on the opposite sides of an organ. VLN2 is preferentially expressed in growing tissues, consistent with a role in regulating cell expansion in developing organs. Biochemically, VLN2 exhibits conserved actin filament bundling, severing and capping activities in vitro, with bundling and stabilizing activity being confirmed in vivo. In line with these findings, the vln2 mutant plants exhibit a more dynamic actin cytoskeleton network than the wild type. We show that vln2 mutant plants exhibit a hypersensitive gravitropic response, faster recycling of PIN2 (an auxin efflux carrier), and altered auxin distribution. Together, our results demonstrate that VLN2 plays an important role in regulating plant architecture by modulating MF dynamics, recycling of PIN2, and polar auxin transport.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000368293600018
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4650]
专题	作物科学研究所_分子生物学系
作者单位	1.Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 4.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Wu, Shengyang,Xie, Yurong,Zhang, Junjie,et al. VLN2 Regulates Plant Architecture by Affecting Microfilament Dynamics and Polar Auxin Transport in Rice[J]. PLANT CELL,2015,27(10):2829-2845.
APA	Wu, Shengyang.,Xie, Yurong.,Zhang, Junjie.,Ren, Yulong.,Zhang, Xin.,...&Wan, Jianmin.(2015).VLN2 Regulates Plant Architecture by Affecting Microfilament Dynamics and Polar Auxin Transport in Rice.PLANT CELL,27(10),2829-2845.
MLA	Wu, Shengyang,et al."VLN2 Regulates Plant Architecture by Affecting Microfilament Dynamics and Polar Auxin Transport in Rice".PLANT CELL 27.10(2015):2829-2845.