Over-expression of an S-domain receptor-like kinase extracellular domain improves panicle architecture and grain yield in rice

doi:10.1093/jxb/erv417

	Over-expression of an S-domain receptor-like kinase extracellular domain improves panicle architecture and grain yield in rice
	Zou, Xiaohua 1; Qin, Zhengrui 1; Zhang, Chunyu 1; Liu, Bin 1; Liu, Jun 1; Zhang, Chengsheng 2; Lin, Chentao 3; Li, Hongyu 1; Zhao, Tao 1
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2015
卷号	66 期号:22 页码:7197-7209
关键词	Panicle architecture rice S-domain receptor-like kinase
ISSN号	0022-0957
DOI	10.1093/jxb/erv417
通讯作者	Zou, Xiaohua
英文摘要	The S-domain receptor kinase (SRK) comprises a highly polymorphic subfamily of receptor-like kinases (RLKs) originally found to be involved in the self-incompatibility response in Brassica. Although several members have been identified to play roles in developmental control and disease responses, the correlation between SRKs and yield components in rice is still unclear. The utility of transgenic expression of a dominant negative form of SRK, OsLSK1 (Large spike S-domain receptor like Kinase 1), is reported here for the improvement of grain yield components in rice. OsLSK1 was highly expressed in nodes of rice and is a plasma membrane protein. The expression of OsLSK1 responded to the exogenous application of growth hormones, to abiotic stresses, and its extracellular domain could form homodimers or heterodimers with other related SRKs. Over-expression of a truncated version of OsLSK1 (including the extracellular and transmembrane domain of OsLSK1 without the intracellular kinase domain) increased plant height and improve yield components, including primary branches per panicle and grains per primary branch, resulting in about a 55.8% increase of the total grain yield per plot (10 plants). Transcriptional analysis indicated that several key genes involved in the GA biosynthetic and signalling pathway were up-regulated in transgenic plants. However, full-length cDNA over-expression and RNAi of OsLSK1 transgenic plants did not exhibit a detectable visual phenotype and possible reasons for this were discussed. These results indicate that OsLSK1 may act redundantly with its homologues to affect yield traits in rice and manipulation of OsLSK1 by the dominant negative method is a practicable strategy to improve grain yield in rice and other crops.
学科主题	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000367815000017
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4626]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Peoples R China 3.Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA
推荐引用方式 GB/T 7714	Zou, Xiaohua,Qin, Zhengrui,Zhang, Chunyu,et al. Over-expression of an S-domain receptor-like kinase extracellular domain improves panicle architecture and grain yield in rice[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(22):7197-7209.
APA	Zou, Xiaohua.,Qin, Zhengrui.,Zhang, Chunyu.,Liu, Bin.,Liu, Jun.,...&Zhao, Tao.(2015).Over-expression of an S-domain receptor-like kinase extracellular domain improves panicle architecture and grain yield in rice.JOURNAL OF EXPERIMENTAL BOTANY,66(22),7197-7209.
MLA	Zou, Xiaohua,et al."Over-expression of an S-domain receptor-like kinase extracellular domain improves panicle architecture and grain yield in rice".JOURNAL OF EXPERIMENTAL BOTANY 66.22(2015):7197-7209.