Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling

doi:10.1093/jxb/erv476

	Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling
	Wang, Wen-Sheng 1; Zhao, Xiu-Qin 1; Li, Min 1,2; Huang, Li-Yu 1; Xu, Jian-Long 1,3; Zhang, Fan 1; Cui, Yan-Ru 1; Fu, Bin-Ying 1,3; Li, Zhi-Kang 1,3
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2016
卷号	67 期号:1 页码:405-419
关键词	ABA metabolomics molecular mechanisms Oryza sativa L salt tolerance transcriptome
ISSN号	0022-0957
DOI	10.1093/jxb/erv476
通讯作者	Wang, Wen-Sheng
英文摘要	To understand the physiological and molecular mechanisms underlying seedling salt tolerance in rice (Oryza sativa L.), the phenotypic, metabolic, and transcriptome responses of two related rice genotypes, IR64 and PL177, with contrasting salt tolerance were characterized under salt stress and salt+abscisic acid (ABA) conditions. PL177 showed significantly less salt damage, lower Na+/K+ ratios in shoots, and Na+ translocation from roots to shoots, attributed largely to better salt exclusion from its roots and salt compartmentation of its shoots. Exogenous ABA was able to enhance the salt tolerance of IR64 by selectively decreasing accumulation of Na+ in its roots and increasing K+ in its shoots. Salt stress induced general and organ-specific increases of many primary metabolites in both rice genotypes, with strong accumulation of several sugars plus proline in shoots and allantoin in roots. This was due primarily to ABA-mediated repression of genes for degradation of these metabolites under salt. In PL177, salt specifically upregulated genes involved in several pathways underlying salt tolerance, including ABA-mediated cellular lipid and fatty acid metabolic processes and cytoplasmic transport, sequestration by vacuoles, detoxification and cell-wall remodeling in shoots, and oxidation-reduction reactions in roots. Combined genetic and transcriptomic evidence shortlisted relatively few candidate genes for improved salt tolerance in PL177.
学科主题	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000367816300030
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4608]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Anhui Agr Univ, Sch Sci, Hefei 230036, Peoples R China 3.Chinese Acad Agr Sci, Shenzhen Inst Breeding & Innovat, Shenzhen 518120, Peoples R China
推荐引用方式 GB/T 7714	Wang, Wen-Sheng,Zhao, Xiu-Qin,Li, Min,et al. Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(1):405-419.
APA	Wang, Wen-Sheng.,Zhao, Xiu-Qin.,Li, Min.,Huang, Li-Yu.,Xu, Jian-Long.,...&Li, Zhi-Kang.(2016).Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling.JOURNAL OF EXPERIMENTAL BOTANY,67(1),405-419.
MLA	Wang, Wen-Sheng,et al."Complex molecular mechanisms underlying seedling salt tolerance in rice revealed by comparative transcriptome and metabolomic profiling".JOURNAL OF EXPERIMENTAL BOTANY 67.1(2016):405-419.