A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties | |
Zhao Pei1; Wang Ke1; Lin Zhi-shan1; Liu Hui-yun1; Li Xin1; Du Li-pu1; Yan Yue-ming2; Ye Xing-guo1 | |
刊名 | JOURNAL OF INTEGRATIVE AGRICULTURE |
2016 | |
卷号 | 15期号:1页码:10-17 |
关键词 | wheat intracultivaral chromosome substitution lines agronomic traits high molecular weight glutenin subunits (HMW-GS) molecular markers |
ISSN号 | 2095-3119 |
DOI | 10.1016/S2095-3119(15)61038-3 |
通讯作者 | Zhao Pei |
英文摘要 | Locating of important agronomic genes onto chromosome is helpful for efficient development of new wheat varieties. Wheat chromosome substitution lines between two varieties have been widely used for locating genes because of their distinctive advantages in genetic analysis, compared with the aneuploid genetic materials. Apart from the substituted chromosome, the other chromosomes between the substitution lines and their recipient parent should be identical, which eases the gene locating practice. In this study, a set of chromosome substitution lines with cv. Wichita (WI) as the recipient parent and cv. Cheyenne (CNN) as the donor parent were studied for the composition of high molecular weight glutenin subunits (HMW-GS) as well as a range of agronomic important traits. Results revealed that the substitution lines of WI(CNN5D), WI(CNN6A) and WI(CNN7B) had higher plant heights than the two parents of WI and CNN, and WI(CNN3D) had later maturity than the parents. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, a substitution line WI(CNN5B) was found to contain different HMW-GS patterns from its two parents, in which 1By9 was replaced by 1By8 on chromosome 1BL. Simple sequence repeat (SSR) analysis confirmed that the variation on 1BL in WI(CNN5B) was originated from Chinese Spring (CS). It is concluded that chromosomal fragments from bridge material and donor parent were quite often retained in intracultivaral chromosome substitution lines except the substituting chromosomes. |
学科主题 | Agriculture, Multidisciplinary |
语种 | 英语 |
出版者 | ELSEVIER SCI LTD |
WOS记录号 | WOS:000368467400002 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/4600] |
专题 | 作物科学研究所_分子生物学系 |
作者单位 | 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Capital Normal Univ, Coll Life Sci, Key Lab Genet & Biotechnol, Beijing 100048, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao Pei,Wang Ke,Lin Zhi-shan,et al. A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(1):10-17. |
APA | Zhao Pei.,Wang Ke.,Lin Zhi-shan.,Liu Hui-yun.,Li Xin.,...&Ye Xing-guo.(2016).A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties.JOURNAL OF INTEGRATIVE AGRICULTURE,15(1),10-17. |
MLA | Zhao Pei,et al."A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties".JOURNAL OF INTEGRATIVE AGRICULTURE 15.1(2016):10-17. |
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