Exogenous auxin regulates multi-metabolic network and embryo development, controlling seed secondary dormancy and germination in Nicotiana tabacum L.

doi:10.1186/s12870-016-0724-5

	Exogenous auxin regulates multi-metabolic network and embryo development, controlling seed secondary dormancy and germination in Nicotiana tabacum L.
	Li, Zhenhua 1,2; Zhang, Jie 2; Liu, Yiling 3; Zhao, Jiehong 2; Fu, Junjie 4; Ren, Xueliang 2; Wang, Guoying 4; Wang, Jianhua 1
刊名	BMC PLANT BIOLOGY
	2016
卷号	16 页码:-
关键词	Auxin Seed dormancy Germination Wateruptake Embryo development Endosperm burst Hormone Nicotiana tabacum RNA-seq
ISSN号	1471-2229
DOI	10.1186/s12870-016-0724-5
通讯作者	Li, Zhenhua
英文摘要	Background: Auxin was recognized as a secondary dormancy phytohormone, controlling seed dormancy and germination. However, the exogenous auxin-controlled seed dormancy and germination remain unclear in physiological process and gene network.Results: Tobacco seeds soaked in 1000 mg/l auxin solution showed markedly decreased germination compared with that in low concentration of auxin solutions and ddH(2)O. Using an electron microscope, observations were made on the seeds which did not unfold properly in comparison to those submerged in ddH(2)O. The radicle traits measured by WinRHIZO, were found to be also weaker than the other treatment groups. Quantified by ELISA, there was no significant difference found in beta-1,3glucanase activity and abscisic acid (ABA) content between the seeds imbibed in gradient concentration of auxin solution and those soaked in ddH(2)O. However, gibberellic acid (GA) and auxin contents were significantly higher at the time of exogenous auxin imbibition and were gradually reduced at germination. RNA sequencing (RNA-seq), revealed that the transcriptome of auxin-responsive dormancy seeds were more similar to that of the imbibed seeds when compared with primary dormancy seeds by principal component analysis. The results of gene differential expression analysis revealed that auxin-controlled seed secondary dormancy was associated with flavonol biosynthetic process, gibberellin metabolic process, adenylyl-sulfate reductase activity, thioredoxin activity, glutamate synthase (NADH) activity and chromatin regulation. In addition, auxin-responsive germination responded to ABA, auxin, jasmonic acid (JA) and salicylic acid (SA) mediated signaling pathway (red, far red and blue light), glutathione and methionine (Met) metabolism.Conclusions: In this study, exogenous auxin-mediated seed secondary dormancy is an environmental model that prevents seed germination in an unfavorable condition. Seeds of which could not imbibe normally, and radicles of which also could not develop normally and emerge. To complete the germination, seeds of which would stimulate more GA synthesis to antagonize the stimulation of exogenous auxin. Exogenous auxin regulates multi-metabolic networks controlling seed secondary dormancy and germination, of which the most important thing was that we found the auxin-responsive seed secondary dormancy refers to epigenetic regulation and germination to enhance Met pathway. Therefore, this study uncovers a previously unrecognized transcriptional regulatory networks and physiological development process of seed dormancy and germination with superfluous auxin signal activate.
学科主题	Plant Sciences
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000369587600001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4577]
专题	作物科学研究所_分子生物学系
作者单位	1.China Agr Univ, Coll Agr & Biotechnol, Yuanmingyuan West Rd, Beijing 100094, Peoples R China 2.Guizhou Acad Tobacco Sci, Mol Genet Key Lab China Tobacco, Guiyang 550081, Peoples R China 3.Guizhou Univ, Inst Tobacco, Guiyang 550025, Peoples R China 4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Li, Zhenhua,Zhang, Jie,Liu, Yiling,et al. Exogenous auxin regulates multi-metabolic network and embryo development, controlling seed secondary dormancy and germination in Nicotiana tabacum L.[J]. BMC PLANT BIOLOGY,2016,16:-.
APA	Li, Zhenhua.,Zhang, Jie.,Liu, Yiling.,Zhao, Jiehong.,Fu, Junjie.,...&Wang, Jianhua.(2016).Exogenous auxin regulates multi-metabolic network and embryo development, controlling seed secondary dormancy and germination in Nicotiana tabacum L..BMC PLANT BIOLOGY,16,-.
MLA	Li, Zhenhua,et al."Exogenous auxin regulates multi-metabolic network and embryo development, controlling seed secondary dormancy and germination in Nicotiana tabacum L.".BMC PLANT BIOLOGY 16(2016):-.