The role of OsMSH4 in male and female gamete development in rice meiosis

doi:10.1093/jxb/erv540

	The role of OsMSH4 in male and female gamete development in rice meiosis
	Wang, Chaolong 1; Wang, Yang 1; Cheng, Zhijun 2; Zhao, Zhigang 1; Chen, Jun 2; Sheng, Peike 2; Yu, Yang 1; Ma, Weiwei 2; Duan, Erchao 1; Wu, Fuqing 2
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2016
卷号	67 期号:5 页码:1447-1459
关键词	Chiasmata Oryza sativa OsMSH4/OsMSH5 heterodimer RPA complex Trisomic
ISSN号	0022-0957
DOI	10.1093/jxb/erv540
通讯作者	Wang, Chaolong
英文摘要	Meiosis is essential for gametogenesis in sexual reproduction in rice (Oryza sativa L.). We identified a MutS-homolog (MSH) family gene OsMSH4 in a trisomic plant. Cytological analysis showed that developments of both pollen and embryo sacs in an Osmsh4 mutant were blocked due to defective chromosome pairing. Compared with the wild type, the Osmsh4 mutant displayed a significant similar to 21.9% reduction in chiasma frequency, which followed a Poisson distribution, suggesting that class I crossover formation in the mutant was impaired. Temporal and spatial expression pattern analyses showed that OsMSH4 was preferentially expressed in meiocytes during their meiosis, indicating a critical role in gametogenesis. Subcellular localization showed that OsMSH4-green fluorescent protein was predominantly located in the nucleus. OsMSH4 could interact with another MSH member (OsMSH5) through the N-terminus and C-terminus, respectively. Direct physical interaction between OsMSH5, OsRPA1a, OsRPA2b, OsRPA1c, and OsRPA2c was identified by yeast two-hybrid assays and further validated by pull-down assays. Our results supported the conclusion that the OsMSH4/5 heterodimer plays a key role in regulation of crossover formation during rice meiosis by interaction with the RPA complex.
学科主题	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000371928000022
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4574]
专题	作物科学研究所_分子生物学系
作者单位	1.Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chaolong,Wang, Yang,Cheng, Zhijun,et al. The role of OsMSH4 in male and female gamete development in rice meiosis[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(5):1447-1459.
APA	Wang, Chaolong.,Wang, Yang.,Cheng, Zhijun.,Zhao, Zhigang.,Chen, Jun.,...&Wan, Jianmin.(2016).The role of OsMSH4 in male and female gamete development in rice meiosis.JOURNAL OF EXPERIMENTAL BOTANY,67(5),1447-1459.
MLA	Wang, Chaolong,et al."The role of OsMSH4 in male and female gamete development in rice meiosis".JOURNAL OF EXPERIMENTAL BOTANY 67.5(2016):1447-1459.