The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures

doi:10.1186/s12284-016-0086-5

	The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures
	Zheng, Kailun 1; Zhao, Jian 1,2; Lin, Dongzhi 1; Chen, Jiaying 1,2; Xu, Jianlong 3; Zhou, Hua 1,4; Teng, Sheng 2; Dong, Yanjun 1
刊名	RICE
	2016
卷号	9 页码:-
关键词	Chloroplast development Deg protease Photosystem Rice
ISSN号	1939-8425
DOI	10.1186/s12284-016-0086-5
通讯作者	Zheng, Kailun
英文摘要	Background: High temperature affects a broad spectrum of cellular components and metabolism in plants. The Deg/HtrA family of ATP-independent serine endopeptidases is present in nearly all organisms. Deg proteases are required for the survival of Escherichia coli at high temperatures. However, it is still unclear whether rice Deg proteases are required for chloroplast development under high temperatures.Results: In this study, we reported the first rice deg mutant tcm5 (thermo-sensitive chlorophyll-deficient mutant 5) that has an albino phenotype, defective chloroplasts and could not survive after the 4-5 leaf seedling stage when grown at high temperature (32 degrees C). However, when grown at low temperatures (20 degrees C), tcm5 has a normal phenotype. Map-based cloning showed that TCM5 encoding a chloroplast-targeted Deg protease protein. The TCM5 transcripts were highly expressed in all green tissues and undetectable in other tissues, showing the tissue-specific expression. In tcm5 mutants grown at high temperatures, the transcript levels of certain genes associated with chloroplast development especially PSII-associated genes were severely affected, but recovered to normal levels at low temperatures. These results showed important role of TCM5 for chloroplast development under high temperatures.Conclusions: The TCM5 encodes chloroplast-targeted Deg protease protein which is important for chloroplast development and the maintenance of PSII function and its disruption would lead to a defective chloroplast and affected expression levels of genes associated with chloroplast development and photosynthesis at early rice seedling stage under high temperatures.
学科主题	Agronomy
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000373080900001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4560]
专题	作物科学研究所_分子生物学系
作者单位	1.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China 3.Chinese Acad Agr Sci, Inst Crop Sci, 12 South Zhong Guan Cun St, Beijing 100081, Peoples R China 4.Hokkaido Univ, Fac Agr, Sappro 0600817, Japan
推荐引用方式 GB/T 7714	Zheng, Kailun,Zhao, Jian,Lin, Dongzhi,et al. The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures[J]. RICE,2016,9:-.
APA	Zheng, Kailun.,Zhao, Jian.,Lin, Dongzhi.,Chen, Jiaying.,Xu, Jianlong.,...&Dong, Yanjun.(2016).The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures.RICE,9,-.
MLA	Zheng, Kailun,et al."The Rice TCM5 Gene Encoding a Novel Deg Protease Protein is Essential for Chloroplast Development under High Temperatures".RICE 9(2016):-.