Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis

doi:10.1186/s12870-016-0806-4

	Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis
	He, Guan-Hua 1; Xu, Ji-Yuan 1; Wang, Yan-Xia 2; Liu, Jia-Ming 1; Li, Pan-Song 1; Chen, Ming 1; Ma, You-Zhi 1; Xu, Zhao-Shi 1
刊名	BMC PLANT BIOLOGY
	2016
卷号	16 页码:-
关键词	Drought tolerance WRKY transcription factor Stress response mechanisms Thermotolerance Triticum aestivum
ISSN号	1471-2229
DOI	10.1186/s12870-016-0806-4
通讯作者	He, Guan-Hua
英文摘要	Background: Drought stress is one of the major causes of crop loss. WRKY transcription factors, as one of the largest transcription factor families, play important roles in regulation of many plant processes, including drought stress response. However, far less information is available on drought-responsive WRKY genes in wheat (Triticum aestivum L.), one of the three staple food crops.Results: Forty eight putative drought-induced WRKY genes were identified from a comparison between de novo transcriptome sequencing data of wheat without or with drought treatment. TaWRKY1 and TaWRKY33 from WRKY Groups III and II, respectively, were selected for further investigation. Subcellular localization assays revealed that TaWRKY1 and TaWRKY33 were localized in the nuclei in wheat mesophyll protoplasts. Various abiotic stress-related cis-acting elements were observed in the promoters of TaWRKY1 and TaWRKY33. Quantitative real-time PCR (qRT-PCR) analysis showed that TaWRKY1 was slightly up-regulated by high-temperature and abscisic acid (ABA), and down-regulated by low-temperature. TaWRKY33 was involved in high responses to high-temperature, low-temperature, ABA and jasmonic acid methylester (MeJA). Overexpression of TaWRKY1 and TaWRKY33 activated several stress-related downstream genes, increased germination rates, and promoted root growth in Arabidopsis under various stresses. TaWRKY33 transgenic Arabidopsis lines showed lower rates of water loss than TaWRKY1 transgenic Arabidopsis lines and wild type plants during dehydration. Most importantly, TaWRKY33 transgenic lines exhibited enhanced tolerance to heat stress.Conclusions: The functional roles highlight the importance of WRKYs in stress response.
学科主题	Plant Sciences
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000377267100001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4520]
专题	作物科学研究所_分子生物学系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci,Minist Agr, Key Lab Biol & Genet Improvement Triticeae Crops, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Shijiazhuang Acad Agr & Forestry Sci, Res Ctr Wheat Engn Technol Hebei, Shijiazhuang 050041, Hebei, Peoples R China
推荐引用方式 GB/T 7714	He, Guan-Hua,Xu, Ji-Yuan,Wang, Yan-Xia,et al. Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis[J]. BMC PLANT BIOLOGY,2016,16:-.
APA	He, Guan-Hua.,Xu, Ji-Yuan.,Wang, Yan-Xia.,Liu, Jia-Ming.,Li, Pan-Song.,...&Xu, Zhao-Shi.(2016).Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis.BMC PLANT BIOLOGY,16,-.
MLA	He, Guan-Hua,et al."Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis".BMC PLANT BIOLOGY 16(2016):-.