Bulked sample analysis in genetics, genomics and crop improvement

doi:10.1111/pbi.12559

	Bulked sample analysis in genetics, genomics and crop improvement
	Zou, Cheng 1; Wang, Pingxi 1; Xu, Yunbi 1,2
刊名	PLANT BIOTECHNOLOGY JOURNAL
	2016
卷号	14 期号:10 页码:1941-1955
关键词	bulked sample analysis bulked segregant analysis microarrays chips DNA-seq RNA-seq protein-seq breeding
ISSN号	1467-7644
DOI	10.1111/pbi.12559
通讯作者	Zou, Cheng
英文摘要	Biological assay has been based on analysis of all individuals collected from sample populations. Bulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked segregant analysis with biparental populations, mapping by sequencing with major gene mutants and pooled genomewide association study using extreme variants. Compared to conventional entire population analysis, BSA significantly reduces the scale and cost by simplifying the procedure. The bulks can be built by selection of extremes or representative samples from any populations and all types of segregants and variants that represent wide ranges of phenotypic variation for the target trait. Methods and procedures for sampling, bulking and multiplexing are described. The samples can be analysed using individual markers, microarrays and high-throughput sequencing at all levels of DNA, RNA and protein. The power of BSA is affected by population size, selection of extreme individuals, sequencing strategies, genetic architecture of the trait and marker density. BSA will facilitate plant breeding through development of diagnostic and constitutive markers, agronomic genomics, marker-assisted selection and selective phenotyping. Applications of BSA in genetics, genomics and crop improvement are discussed with their future perspectives.
学科主题	Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000383625100001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105315]
专题	作物科学研究所_遗传育种系
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing, Peoples R China 2.Int Maize & Wheat Improvement Ctr CIMMYT, Texcoco, Mexico
推荐引用方式 GB/T 7714	Zou, Cheng,Wang, Pingxi,Xu, Yunbi. Bulked sample analysis in genetics, genomics and crop improvement[J]. PLANT BIOTECHNOLOGY JOURNAL,2016,14(10):1941-1955.
APA	Zou, Cheng,Wang, Pingxi,&Xu, Yunbi.(2016).Bulked sample analysis in genetics, genomics and crop improvement.PLANT BIOTECHNOLOGY JOURNAL,14(10),1941-1955.
MLA	Zou, Cheng,et al."Bulked sample analysis in genetics, genomics and crop improvement".PLANT BIOTECHNOLOGY JOURNAL 14.10(2016):1941-1955.