Transcriptome profiling and comparison of maize ear heterosis during the spikelet and floret differentiation stages

doi:10.1186/s12864-016-3296-8

	Transcriptome profiling and comparison of maize ear heterosis during the spikelet and floret differentiation stages
	Hu, Xiaojiao; Wang, Hongwu; Diao, Xizhou; Liu, Zhifang; Li, Kun; Wu, Yujin; Liang, Qianjin; Wang, Hui; Huang, Changling
刊名	BMC GENOMICS
	2016
卷号	17 页码:-
关键词	Maize (Zea mays L.) Ear development Heterosis Transcriptomics Additive gene expression Allele-specific expression cis- and trans-regulation
ISSN号	1471-2164
DOI	10.1186/s12864-016-3296-8
通讯作者	Hu, Xiaojiao
英文摘要	Background: Hybridization is a prominent process in the evolution of crop plants that can give rise to gene expression variation, phenotypic novelty and heterosis. Maize is the most successful crop in utilizing heterosis. The development of hybrid maize ears exhibits strong heterotic vigor and greatly affects maize yield. However, a comprehensive perspective on transcriptional variation and its correlation with heterosis during maize ear development is not available.Results: Using RNA sequencing technology, we investigated the transcriptome profiles of maize ears in the spikelet and floret differentiation stages of hybrid ZD808 and its parents CL11 and NG5. Our results revealed that 53.9% ( 21,258) of maize protein-coding genes were transcribed in at least one genotype. In both development stages, significant numbers of genes were differentially expressed between the hybrid and its parents. Gene expression inheritance analysis revealed approximately 80% of genes were expressed additively, which suggested that the complementary effect may play a foundation role in maize ear heterosis. Among non-additively expressed genes, NG5-dominant genes were predominant. Analyses of the allele-specific gene expression in hybrid identified pervasive allelic imbalance and significant preferential expression of NG5 alleles in both developmental stages. The results implied that NG5 may provide beneficial alleles that contribute greatly to heterosis. Further comparison of parental and hybrid allele-specific expression suggested that gene expression variation is largely attributable to cis-regulatory variation in maize. The cis-regulatory variations tend to preserve the allelic expression levels in hybrid and result in additive expression. Comparison between the two development stages revealed that allele-specific expression and cis-/trans-regulatory variations responded differently to developmental cues, which may lead to stage-specific vigor phenotype during maize ear development.Conclusion: Our research suggests that cis-regulated additive expression may fine-tune gene expression level into an optimal status and play a foundation role in maize ear heterosis. Our work provides a comprehensive insight into transcriptional variation and its correlation with heterosis during maize ear development. The knowledge gained from this study presents novel opportunity to improve our maize varieties.
学科主题	Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000388773100007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105287]
专题	作物科学研究所_遗传育种系
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, 12 Zhongguancun South Main St, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Hu, Xiaojiao,Wang, Hongwu,Diao, Xizhou,et al. Transcriptome profiling and comparison of maize ear heterosis during the spikelet and floret differentiation stages[J]. BMC GENOMICS,2016,17:-.
APA	Hu, Xiaojiao.,Wang, Hongwu.,Diao, Xizhou.,Liu, Zhifang.,Li, Kun.,...&Huang, Changling.(2016).Transcriptome profiling and comparison of maize ear heterosis during the spikelet and floret differentiation stages.BMC GENOMICS,17,-.
MLA	Hu, Xiaojiao,et al."Transcriptome profiling and comparison of maize ear heterosis during the spikelet and floret differentiation stages".BMC GENOMICS 17(2016):-.