Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)

doi:10.1016/j.jcs.2007.04.013

	Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)
	Zhang, Qian 1; Dong, Yanmin 1; An, Xueli 1; Wang, Aili 1; Zhang, Yanzhen 1; Li, Xiaohui 1; Gao, Liyan 1; Xia, Xianchun 2,3; He, Zhonghu 2,3; Yan, Yueming 1
刊名	JOURNAL OF CEREAL SCIENCE
	2008
卷号	47 期号:2 页码:252-261
关键词	HMW-GS molecular weight mass spectrometry PTM
ISSN号	0733-5210
DOI	10.1016/j.jcs.2007.04.013
通讯作者	Zhang, Qian
英文摘要	The sample preparation method of high molecular weight glutenin subunits (HMW-GS) for matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, without a separation step, by high-performance liquid chromatography (HPLC) was established in this study. Three major factors influencing mass spectra-the ratio of components of the solvent, the resolving time, and the sample volume-were optimized using HMW-GS mixtures extracted from Chinese cultivar Jing 411. The results showed that the optimized method for sample preparation was to resolve HMW-GS from 20 mg in an hour with 50 mu 1 solvent of 0.4% TFA, 30.0% ACN and 69.6% H2O. The stable mass spectra and accurate molecular weights of 16 major HMW glutenin subunits from common wheat and related species were obtained using the optimized MALDI-TOF-MS method. Seven subunits, where each was from 2-5 cultivars, showed very similar molecular weights. The determined molecular weights of I I subunits were close to those calculated from their coding sequences. In addition, no positive reaction between HMW-GS and GelCode((R)) Glycoprotein Staining Reagent was observed. These results suggested that HMW-GS lack extensive post-translational modifications (PTMs), but low levels of glycosylation or phosphorylation present in some subunits cannot be ruled out. Because of its ability to obtain a rapid, complete and precise profile of HMW glutenin subunits without purifying procedures, MALDI-TOF-MS is expected to be a powerful technique for structural and functional studies of HMW glutenin subunits as well as other cereal proteins. (c) 2007 Elsevier Ltd. All rights reserved.
学科主题	Food Science & Technology ; FOOD SCIENCE & TECHNOLOGY
语种	英语
出版者	ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
WOS记录号	WOS:000254723200015
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5621]
专题	作物科学研究所_遗传育种系
作者单位	1.Capital Normal Univ, Key Lab Genet & Biotechnol, Coll Life Sci, Beijing 100037, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Beijing 100081, Peoples R China 3.Chinese Acad Agr Sci, CIMMYT, China Off, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qian,Dong, Yanmin,An, Xueli,et al. Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)[J]. JOURNAL OF CEREAL SCIENCE,2008,47(2):252-261.
APA	Zhang, Qian.,Dong, Yanmin.,An, Xueli.,Wang, Aili.,Zhang, Yanzhen.,...&Yan, Yueming.(2008).Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).JOURNAL OF CEREAL SCIENCE,47(2),252-261.
MLA	Zhang, Qian,et al."Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)".JOURNAL OF CEREAL SCIENCE 47.2(2008):252-261.