Development of Functional Markers for a Lipoxygenase Gene TaLox-B1 on Chromosome 4BS in Common Wheat | |
Geng, Hongwei1,2; Xia, Xianchun1; Zhang, Liping3; Qu, Yanying2; He, Zhonghu1,4 | |
刊名 | CROP SCIENCE |
2012 | |
卷号 | 52期号:2页码:568-576 |
ISSN号 | 0011-183X |
DOI | 10.2135/cropsci2011.07.0365 |
通讯作者 | Geng, Hongwei |
英文摘要 | Lipoxygenase (LOX) activity in grain influences the color and processing quality of wheat (Triticum spp.)-based products. Characterization of LOX genes and development of functional markers are of importance for marker-assisted selection in common wheat (Triticum aestivum L.) breeding. In the present study, the full-length genomic DNA (gDNA) sequence of a LOX gene (designated TaLox-B1) located on chromosome 4BS was characterized by in silico cloning and experimental validation. Two complementary dominant sequence tagged site (STS) markers, LOX16 and LOX18, were developed based on the single nucleotide polymorphism (SNP) of two alleles at the TaLox-B1 locus, amplifying 489- and 791-bp fragments in cultivars with higher and lower LOX activities, respectively. The two markers were mapped on chromosome 4BS using a doubled haploid (DH) population derived from Zhongyou 9507 x CA9632, a set of Chinese Spring nullisomic-tetrasomic lines, ditelosomic line 4BS, and Langdon (LDN) 4D(4B) chromosome substitution line. Quantitative trait loci (OIL) analysis indicated that TaLox-B1 co-segregated with the two functional markers and was closely linked to simple sequence repeat (SSR) locus Xgwm251 on chromosome 4BS with a genetic distance of 1.8 cM. LOX16 and LOX18 were validated on 198 Chinese wheat cultivars and advanced lines and showed highly significant (p < 0.01) associations with LOX activity. These results suggested that LOX16 and LOX18, co-segregating with TaLox-B1, could be used for the improvement of color attributes of noodles and other wheat-based products in wheat breeding programs. |
学科主题 | Agronomy ; AGRONOMY |
语种 | 英语 |
出版者 | CROP SCIENCE SOC AMER |
WOS记录号 | WOS:000300800400011 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/5189] |
专题 | 作物科学研究所_遗传育种系 |
作者单位 | 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 2.Xinjiang Agr Univ, Coll Agron, Urumqi 830052, Xinjiang, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Beijing Engn & Tech Res Ctr Hybrid Wheat, Beijing 100097, Peoples R China 4.Chinese Acad Agr Sci, Int Maize & Wheat Improvement Ctr CIMMYT China Of, Beijing 100081, Peoples R China |
推荐引用方式 GB/T 7714 | Geng, Hongwei,Xia, Xianchun,Zhang, Liping,et al. Development of Functional Markers for a Lipoxygenase Gene TaLox-B1 on Chromosome 4BS in Common Wheat[J]. CROP SCIENCE,2012,52(2):568-576. |
APA | Geng, Hongwei,Xia, Xianchun,Zhang, Liping,Qu, Yanying,&He, Zhonghu.(2012).Development of Functional Markers for a Lipoxygenase Gene TaLox-B1 on Chromosome 4BS in Common Wheat.CROP SCIENCE,52(2),568-576. |
MLA | Geng, Hongwei,et al."Development of Functional Markers for a Lipoxygenase Gene TaLox-B1 on Chromosome 4BS in Common Wheat".CROP SCIENCE 52.2(2012):568-576. |
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