Root-lodging resistance in maize as an example for high-throughput genetic mapping via single nucleotide polymorphism-based selective genotyping

doi:10.1111/pbr.12010

	Root-lodging resistance in maize as an example for high-throughput genetic mapping via single nucleotide polymorphism-based selective genotyping
	Farkhari, Mohammad 1,2,3; Krivanek, Alan 3; Xu, Yunbi 3,4; Rong, Tingzhao 5; Naghavi, Mohammad R.1; Samadi, Bahman Y.1; Lu, Yanli 3,5
刊名	PLANT BREEDING
	2013
卷号	132 期号:1 页码:90-98
关键词	maize selective genotyping root lodging quantitative trait loci segregation distortion regions high-throughput genetic mapping
ISSN号	0179-9541
DOI	10.1111/pbr.12010
通讯作者	Farkhari, Mohammad
英文摘要	Large-scale selective genotyping and high-throughput analysis are two important strategies for low-cost and high-effective genetic mapping. In this study, selective genotyping was applied to four maize F2 populations. Thirty plants were selected from each of the two tails of the original F2 populations to represent extreme resistant and susceptible plants to root lodging, and genotyped individually with 1536 single nucleotide polymorphisms (SNPs). A quantitative trait locus (QTL) was declared when at least three closely linked SNPs showed significant allele frequency difference between the two tails. Nine QTL were identified for root lodging across the four populations, which were located on chromosomes 2, 4, 5, 7, 8 and 10 and one of them was shared between two populations. A total of 20 segregation distortion regions (SDRs) were identified across the four populations, one of which was co-localized with a QTL on chromosome 4. The tightly linked SNPs identified in this study can be used for marker-assisted selection for root lodging. Selective genotyping, when combined with pooled DNA analysis, can be used to develop strategies for high-throughput genetic mapping for all crops.
学科主题	Agronomy ; Biotechnology & Applied Microbiology ; Plant Sciences ; AGRONOMY ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000313893100012
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5074]
专题	作物科学研究所_遗传育种系
作者单位	1.Univ Tehran, Coll Agr, Agron & Plant Breeding Dept, Karaj, Iran 2.Ramin Univ Agr & Nat Resources, Plant Breeding & Biotechnol Dept, Khouzestan, Iran 3.Int Maize & Wheat Improvement Ctr CIMMYT, El Batan, Texcoco, Mexico 4.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100193, Peoples R China 5.Sichuan Agr Univ, Maize Res Inst, Wenjiang, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Farkhari, Mohammad,Krivanek, Alan,Xu, Yunbi,et al. Root-lodging resistance in maize as an example for high-throughput genetic mapping via single nucleotide polymorphism-based selective genotyping[J]. PLANT BREEDING,2013,132(1):90-98.
APA	Farkhari, Mohammad.,Krivanek, Alan.,Xu, Yunbi.,Rong, Tingzhao.,Naghavi, Mohammad R..,...&Lu, Yanli.(2013).Root-lodging resistance in maize as an example for high-throughput genetic mapping via single nucleotide polymorphism-based selective genotyping.PLANT BREEDING,132(1),90-98.
MLA	Farkhari, Mohammad,et al."Root-lodging resistance in maize as an example for high-throughput genetic mapping via single nucleotide polymorphism-based selective genotyping".PLANT BREEDING 132.1(2013):90-98.