EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

doi:10.5586/asbp.3502

	EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.
	Zhang, Caiyun 1; Shi, Wenshuo 1; Ma, Keshi 1; Li, Hongjie 2; Zhang, Feixiong 1
刊名	ACTA SOCIETATIS BOTANICORUM POLONIAE
	2016
卷号	85 期号:3 页码:-
关键词	Ca2+ deprivation EGTA genomic DNA damage DNA methylation RAPD CRED-RA Triticum aestivum L.
ISSN号	0001-6977
DOI	10.5586/asbp.3502
通讯作者	Zhang, Caiyun
英文摘要	In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly down-regulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca2+ chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.
学科主题	Plant Sciences
语种	英语
出版者	POLSKIE TOWARZYSTWO BOTANICZNE
WOS记录号	WOS:000385711100003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105353]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Capital Normal Univ, Coll Life Sci, 105 Xi San Huan Bei Lu, Beijing 100048, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Caiyun,Shi, Wenshuo,Ma, Keshi,et al. EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.[J]. ACTA SOCIETATIS BOTANICORUM POLONIAE,2016,85(3):-.
APA	Zhang, Caiyun,Shi, Wenshuo,Ma, Keshi,Li, Hongjie,&Zhang, Feixiong.(2016).EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L..ACTA SOCIETATIS BOTANICORUM POLONIAE,85(3),-.
MLA	Zhang, Caiyun,et al."EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.".ACTA SOCIETATIS BOTANICORUM POLONIAE 85.3(2016):-.