Development and characterization of chromosome segment substitution lines derived from Oryza rufipogon in the genetic background of O. sativa spp. indica cultivar 9311

doi:10.1186/s12864-016-2987-5

	Development and characterization of chromosome segment substitution lines derived from Oryza rufipogon in the genetic background of O. sativa spp. indica cultivar 9311
	Qiao, Weihua 1; Qi, Lan 1,2; Cheng, Zhijun 1; Su, Long 1; Li, Jing 1; Sun, Yan 1; Ren, Junfang 3; Zheng, Xiaoming 1; Yang, Qingwen 1
刊名	BMC GENOMICS
	2016
卷号	17 页码:-
关键词	Chromosome segment substitution lines Oryza rufipogon Quantitative trait locus
ISSN号	1471-2164
DOI	10.1186/s12864-016-2987-5
通讯作者	Qiao, Weihua
英文摘要	Background: Wild rice (Oryza rufipogon) constitutes a primary gene source for rice breed improvement. Chromosome segment substitution line (CSSL) for O. rufipogon is a powerful tool for fine mapping of quantitative traits, new gene discovery, and marker-assisted breeding. Thus, they provide a basis for a wide range of genomic and genetic studies.Results: In this study, a set of 198 CSSLs were developed from a cross between recurrent parent indica var. 9311 and an O. rufipogon donor parent; these were then genotyped using 313 polymorphic SSR markers evenly distributed across the 12 rice chromosomes. On average, each CSSL carried 2.16 introgressed segments, and the genetic distance of each segment was about 6 cM. The segments collectively covered 84.9 % of the wild rice genome. Based on these CSSLs, 25 QTLs involved in 10 agronomic traits were identified. Seven CSSLs were subjected to a whole-genome single nucleotide polymorphism chip assay and two QTLs, qSH4-1 and qDTH10-1, detected. In addition, a new QTL associated with the heading date was detected in a 78-Kb region on chromosome 10, thus proving the ability of these CSSLs to identify new QTLs and genes.Conclusions: The newly developed CSSL population proved a useful tool for both gene identification and whole-genome research of wild rice. These CSSL materials will provide a foundation for rice variety improvement.
学科主题	Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000381226400005
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105333]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, 12 Zhongguancun South St, Beijing 100081, Haidian, Peoples R China 2.Hainan Acad Agr Sci, Inst Cereal Crop Sci, 14 Xingdan Rd, Haikou 571100, Hainan, Peoples R China 3.Hainan Acad Agr Sci, Inst Trop Hort, 14 Xingdan Rd, Haikou 571100, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Weihua,Qi, Lan,Cheng, Zhijun,et al. Development and characterization of chromosome segment substitution lines derived from Oryza rufipogon in the genetic background of O. sativa spp. indica cultivar 9311[J]. BMC GENOMICS,2016,17:-.
APA	Qiao, Weihua.,Qi, Lan.,Cheng, Zhijun.,Su, Long.,Li, Jing.,...&Yang, Qingwen.(2016).Development and characterization of chromosome segment substitution lines derived from Oryza rufipogon in the genetic background of O. sativa spp. indica cultivar 9311.BMC GENOMICS,17,-.
MLA	Qiao, Weihua,et al."Development and characterization of chromosome segment substitution lines derived from Oryza rufipogon in the genetic background of O. sativa spp. indica cultivar 9311".BMC GENOMICS 17(2016):-.