DEVELOPMENT AND CHARACTERIZATION OF 20 NOVEL POLYMORPHIC STS MARKERS IN VICIA FABA (FAVA BEAN)

doi:10.3732/ajb.1100092

	DEVELOPMENT AND CHARACTERIZATION OF 20 NOVEL POLYMORPHIC STS MARKERS IN VICIA FABA (FAVA BEAN)
	Wang, Haifei; Yang, Tao; Guan, Jianping; Ma, Yu; Sun, Xuelian; Zong, Xuxiao
刊名	AMERICAN JOURNAL OF BOTANY
	2011
卷号	98 期号:7 页码:E189-E191
关键词	polymorphism primers sequence tagged site (STS) Vicia faba
ISSN号	0002-9122
DOI	10.3732/ajb.1100092
通讯作者	Wang, Haifei
英文摘要	Premise of the study: Sequence tagged site (STS) primers were developed for Vicia faba, based on amplified bands by ISSR primers. The usefulness of these STS markers was validated for size polymorphism among fava bean accessions.Methods and Results: Based on the sequences derived from intersimple sequence repeat (ISSR) amplifi cation, 66 sequence tagged site (STS) primer pairs were developed and screened, and 20 of them were polymorphic. The polymorphism of these markers was identified in 32 fava bean germplasm from different global geographical locations. Alleles (N(a)) per locus numbered 2 to 4, and expected heterozygosity (H(E)) per locus ranged from 0.000 to 0.714. There was significant variation in H(E) among germplasm from different regions for individual primers.Conclusions: These novel polymorphic STS markers may be useful and convenient for further studies of population genetics, cultivar identification, and evolution in fava bean.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	BOTANICAL SOC AMER INC
WOS记录号	WOS:000292377000009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5294]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Haifei,Yang, Tao,Guan, Jianping,et al. DEVELOPMENT AND CHARACTERIZATION OF 20 NOVEL POLYMORPHIC STS MARKERS IN VICIA FABA (FAVA BEAN)[J]. AMERICAN JOURNAL OF BOTANY,2011,98(7):E189-E191.
APA	Wang, Haifei,Yang, Tao,Guan, Jianping,Ma, Yu,Sun, Xuelian,&Zong, Xuxiao.(2011).DEVELOPMENT AND CHARACTERIZATION OF 20 NOVEL POLYMORPHIC STS MARKERS IN VICIA FABA (FAVA BEAN).AMERICAN JOURNAL OF BOTANY,98(7),E189-E191.
MLA	Wang, Haifei,et al."DEVELOPMENT AND CHARACTERIZATION OF 20 NOVEL POLYMORPHIC STS MARKERS IN VICIA FABA (FAVA BEAN)".AMERICAN JOURNAL OF BOTANY 98.7(2011):E189-E191.