Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar

doi:10.1007/s00122-013-2073-1

	Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar
	Zhang, Jiqing; Xia, Changjian; Wang, Xiaoming; Duan, Canxing; Sun, Suli; Wu, Xiaofei; Zhu, Zhendong
刊名	THEORETICAL AND APPLIED GENETICS
	2013
卷号	126 期号:6 页码:1555-1561
ISSN号	0040-5752
DOI	10.1007/s00122-013-2073-1
通讯作者	Zhang, Jiqing
英文摘要	Phytophthora root rot (PRR), caused by Phytophthora sojae Kaufmann & Gerdemann, is one of the most destructive diseases of soybean [Glycine max (L.) Merr.]. Deployment of resistance genes is the most economical and effective way of controlling the disease. The soybean cultivar 'Yudou 29' is resistant to many P. sojae isolates in China. The genetic basis of the resistance in 'Yudou 29' was elucidated through an inheritance study and molecular mapping. In response to 25 P. sojae isolates, 'Yudou 29' displayed a new resistance reaction pattern distinct from those of differentials carrying known Rps genes. A population of 214 F-2:3 families from a cross between 'Jikedou 2' (PRR susceptible) and 'Yudou 29' was used for Rps gene mapping. The segregation fit a ratio of 1:2:1 for resistance:segregation:susceptibility within this population, indicating that resistance in 'Yudou 29' is controlled by a single dominant gene. This gene was temporarily named RpsYD29 and mapped on soybean chromosome 03 (molecular linkage group N; MLG N) flanked by SSR markers SattWM82-50 and Satt1k4b at a genetic distance of 0.5 and 0.2 cM, respectively. Two nucleotide binding site-leucine rich repeat (NBS-LRR) type genes were detected in the 204.8 kb region between SattWM82-50 and Satt1k4b. These two genes showed high similarity to Rps1k in amino acid sequence and could be candidate genes for PRR resistance. Based on the phenotype reactions and the physical position on soybean chromosome 03, RpsYD29 might be a novel allele at, or a novel gene tightly linked to, the Rps1 locus.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000319477900012
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5036]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, MOA Key Lab Soybean Biol Beijing, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jiqing,Xia, Changjian,Wang, Xiaoming,et al. Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar[J]. THEORETICAL AND APPLIED GENETICS,2013,126(6):1555-1561.
APA	Zhang, Jiqing.,Xia, Changjian.,Wang, Xiaoming.,Duan, Canxing.,Sun, Suli.,...&Zhu, Zhendong.(2013).Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar.THEORETICAL AND APPLIED GENETICS,126(6),1555-1561.
MLA	Zhang, Jiqing,et al."Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar".THEORETICAL AND APPLIED GENETICS 126.6(2013):1555-1561.