Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance

doi:10.1371/journal.pone.0132314

	Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance
	Wu, Jinxia 1; Zhang, Zhiguo 1; Zhang, Qian 1; Liu, Yayun 1; Zhu, Butuo 1; Cao, Jian 2; Li, Zhanpeng 1; Han, Longzhi 2; Jia, Jizeng 2; Zhao, Guangyao 2
刊名	PLOS ONE
	2015
卷号	10 期号:7 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0132314
通讯作者	Wu, Jinxia
英文摘要	Transcription factors (TFs) play important roles in plant growth, development, and responses to environmental stress. In this study, we collected 1,455 full-length (FL) cDNAs of TFs, representing 45 families, from wheat and its relatives Triticum urartu, Aegilops speltoides, Aegilops tauschii, Triticum carthlicum, and Triticum aestivum. More than 15,000 T-0 TF FOX (Full-length cDNA Over-eXpressing) rice lines were generated; of these, 10,496 lines set seeds. About 14.88% of the T-0 plants showed obvious phenotypic changes. T-1 lines (5,232 lines) were screened for salt and osmotic stress tolerance using 150 mM NaCl and 20% (v/v) PEG-4000, respectively. Among them, five lines (591, 746, 1647, 1812, and J4065) showed enhanced salt stress tolerance, five lines (591, 746, 898, 1078, and 1647) showed enhanced osmotic stress tolerance, and three lines (591, 746, and 1647) showed both salt and osmotic stress tolerance. Further analysis of the T-DNA flanking sequences showed that line 746 over-expressed TaEREB1, line 898 over-expressed TabZIPD, and lines 1812 and J4065 over-expressed TaOBF1a and TaOBF1b, respectively. The enhanced salt and osmotic stress tolerance of lines 898 and 1812 was confirmed by retransformation of the respective genes. Our results demonstrate that a heterologous FOX system may be used as an alternative genetic resource for the systematic functional analysis of the wheat genome.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000358197600099
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4691]
专题	作物科学研究所_种质资源保存与研究中心
作者单位	1.Chinese Acad Agr Sci, Biotechnol Res Inst, Natl Key Facil Gene Resources & Gene Improvement, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Chinese Natl Key Facil Gene Resources & Gene Impr, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wu, Jinxia,Zhang, Zhiguo,Zhang, Qian,et al. Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance[J]. PLOS ONE,2015,10(7):-.
APA	Wu, Jinxia.,Zhang, Zhiguo.,Zhang, Qian.,Liu, Yayun.,Zhu, Butuo.,...&Sun, Xuehui.(2015).Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance.PLOS ONE,10(7),-.
MLA	Wu, Jinxia,et al."Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance".PLOS ONE 10.7(2015):-.