GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress

doi:10.1007/s11103-013-0040-5

CORC > 中国农业科学院 > 作物科学研究所 > 职能部门

	GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress
	Ni, Zhiyong; Hu, Zheng; Jiang, Qiyan; Zhang, Hui
刊名	PLANT MOLECULAR BIOLOGY
	2013
卷号	82 期号:1-2 页码:113-129
关键词	GmNFYA3 Drought tolerance Soybean miR169 ABA and salt sensitivity
ISSN号	0167-4412
DOI	10.1007/s11103-013-0040-5
通讯作者	Ni, Zhiyong
英文摘要	Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by abscisic acid (ABA) and abiotic stresses, such as polyethylene glycol, NaCl and cold. Subcellular localization analysis suggested that GmNFYA3 may activate its specific targets in the nucleus. Histochemical beta-glucuronidase (GUS) staining revealed that the expression of the GUS gene driven by the GmNFYA3 promoter occurred in various transgenic Arabidopsis tissues. Coexpression in Nicotiana benthamiana and 5' RACE assays indicated that miR169 directs GmNFYA3 mRNA cleavage in vivo. Overexpression of GmNFYA3 resulted in Arabidopsis with reduced leaf water loss and enhanced drought tolerance. In addition, the transgenic Arabidopsis exhibited increased sensitivity to high salinity and exogenous ABA. Moreover, the transcript levels of ABA biosynthesis (ABA1, ABA2), ABA signaling (ABI1, ABI2) and stress-responsive genes, including RD29A and CBF3, were generally higher in GmNFYA3 plants than in wild-type controls under normal conditions. These results suggest that the GmNFYA3 gene functions in positive modulation of drought stress tolerance and has potential applications in molecular breeding to enhance drought tolerance in crops.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000318798900009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5045]
专题	作物科学研究所_职能部门
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Ni, Zhiyong,Hu, Zheng,Jiang, Qiyan,et al. GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress[J]. PLANT MOLECULAR BIOLOGY,2013,82(1-2):113-129.
APA	Ni, Zhiyong,Hu, Zheng,Jiang, Qiyan,&Zhang, Hui.(2013).GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress.PLANT MOLECULAR BIOLOGY,82(1-2),113-129.
MLA	Ni, Zhiyong,et al."GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress".PLANT MOLECULAR BIOLOGY 82.1-2(2013):113-129.