Isolation and Characterization of Mlo and NBS-LRR-like Gene Sequences in Wheat

CORC > 中国农业科学院 > 作物科学研究所

	Isolation and Characterization of Mlo and NBS-LRR-like Gene Sequences in Wheat
	Li Aili 1; Kong Xiuying 1; Zhou Ronghua 1; Ma Zhiying 2; Jia Jizeng 1
刊名	Acta Botanica Sinica
	2003
卷号	45 期号:4 页码:472-478
关键词	Mlo NBS-LRR cDNA cloning NIL RGA
ISSN号	1672-6650
其他题名	小麦Mlo及NBS-LRR类抗病基因同源序列的分离与鉴定
英文摘要	Based on both cDNA sequence of barley powdery mildew resistance control element Mlo and DNA sequence of the known putative disease resistance gene from Triticum monococcum L., we designed some primers to amplify resistant homologous sequences in the near isogenic lines (NILs) of powdery mildew resistance using RT-PCR method. Two expressed cDNA fragments were isolated from wheat genome. One showed 83% homology to the Mlo gene of barley. The other contained two possible open reading frames (ORFs). NBS conservative domains 2, 3 of disease resistance gene and 13 LRR structures similar to rice Pib protein terminal were found respectively in the two ORFs. It indicated that the latter fragment belongs to NBS-LRR-like genes. The obvious difference of RT-PCR products was observed between the before challenged and the challenged for 72 h by Blumeria graminis f. sp. tritici, which implied that this sequence could be associated with disease resistance of wheat. Using nulli-tetrasomic lines of "Chinese Spring", the NBS-LRR-like gene had been located on chromosome 1D.
学科主题	植物学
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/106455]
专题	作物科学研究所
作者单位	1.Institute of Crop Germplasm Resources, The Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, 北京, 100081 2.College of Agriculture, Hebei Agricultural University, Baoding, 071001
推荐引用方式 GB/T 7714	Li Aili,Kong Xiuying,Zhou Ronghua,et al. Isolation and Characterization of Mlo and NBS-LRR-like Gene Sequences in Wheat[J]. Acta Botanica Sinica,2003,45(4):472-478.
APA	Li Aili,Kong Xiuying,Zhou Ronghua,Ma Zhiying,&Jia Jizeng.(2003).Isolation and Characterization of Mlo and NBS-LRR-like Gene Sequences in Wheat.Acta Botanica Sinica,45(4),472-478.
MLA	Li Aili,et al."Isolation and Characterization of Mlo and NBS-LRR-like Gene Sequences in Wheat".Acta Botanica Sinica 45.4(2003):472-478.