Resistance to powdery mildew in the pea cultivar Xucai 1 is conferred by the gene er1

doi:10.1016/j.cj.2015.07.006

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	Resistance to powdery mildew in the pea cultivar Xucai 1 is conferred by the gene er1
	Sun Suli; Wang Zhongyi; Fu Haining; Duan Canxing; Wang Xiaoming; Zhu Zhendong
刊名	The Crop Journal
	2015
卷号	3 期号:6 页码:489-499
关键词	Erysiphe pisi er1-2 Pea Powdery mildew Xucai 1
ISSN号	2095-5421
DOI	10.1016/j.cj.2015.07.006
英文摘要	Powdery mildew, caused by Erysiphe pisi D.C., is a major constraint to pea production worldwide. The pea cultivar Xucai 1 has shown high resistance to E. pisi under greenhouse and field conditions. The objectives of this study were to identify and characterize genes conferring resistance to powdery mildew in Xucai 1. Three crosses, Qizhen 76 ?Xucai 1, Bawan 6 ?Xucai 1, and Xucai 1 ?Bawan 6, were made to generate populations for genetic analysis. The resistance to E. pisi and segregation ratios in the F_1, F_2, and F_(2:3) populations suggested a single recessive gene conferring the resistance of Xucai 1. Bulked segregant analysis was used to map the resistance gene using two F_2 populations. The resistance gene was close to markers AD60 and c5DNAmet on linkage group VI with genetic distances of 9.9 cM and 15.4 cM in the Xucai 1 ?Bawan 6 F_2 population and 8.7 cM and 8.1 cM in the Qizhen 76 ?Xucai 1 F_2 population, respectively, suggesting that the resistance gene was an er1 allele. This hypothesis was confirmed by comparison of the cDNA sequences of the PsMLO1 gene between the parents and the PsMLO1 wild type. Three distinct types of transcripts in Xucai 1, characterized by a 129-bp deletion and 155- and 220-bp insertions, were detected, consistent with the structure of the er1-2 allele. We concluded that resistance in Xucai 1 was conferred by er1-2 and that its linked markers will be useful in pea breeding programs.
学科主题	园艺
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/106068]
专题	作物科学研究所
作者单位	Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, 100081
推荐引用方式 GB/T 7714	Sun Suli,Wang Zhongyi,Fu Haining,et al. Resistance to powdery mildew in the pea cultivar Xucai 1 is conferred by the gene er1[J]. The Crop Journal,2015,3(6):489-499.
APA	Sun Suli,Wang Zhongyi,Fu Haining,Duan Canxing,Wang Xiaoming,&Zhu Zhendong.(2015).Resistance to powdery mildew in the pea cultivar Xucai 1 is conferred by the gene er1.The Crop Journal,3(6),489-499.
MLA	Sun Suli,et al."Resistance to powdery mildew in the pea cultivar Xucai 1 is conferred by the gene er1".The Crop Journal 3.6(2015):489-499.