Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22

doi:10.1007/s11032-016-0437-3

CORC > 中国农业科学院 > 作物科学研究所

	Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22
	Wang, Cuifen 1; Yin, Guihong 2; Xia, Xianchun 3; He, Zhonghu 4; Zhang, Peipei 1; Yao, Zhanjun 1; Qin, Jinyan 1; Li, Zaifeng 1; Liu, Daqun 1
刊名	MOLECULAR BREEDING
	2016
卷号	36 期号:2 页码:-
关键词	Common wheat Molecular markers Puccinia triticina Leaf rust Molecular mapping
ISSN号	1380-3743
DOI	10.1007/s11032-016-0437-3
通讯作者	Wang, Cuifen
英文摘要	Leaf rust, caused by Puccinia triticina, is one of the most widespread diseases in common wheat globally. The Chinese wheat cultivar Zhoumai 22 is highly resistant to leaf rust at the seedling and adult stages. Seedlings of Zhoumai 22 and 36 lines with known leaf rust resistance genes were inoculated with 13 P. triticina races for gene postulation. The leaf rust response of Zhoumai 22 was different from those of the single gene lines. With the objective of identifying and mapping, the new gene(s) for resistance to leaf rust, F-1, F-2 plants and F-2:3 lines from the cross Zhoumai 22/Chinese Spring were inoculated with Chinese P. triticina race FHDQ at the seedling stage. A single dominant gene, tentatively designated LrZH22, conferred resistance. To identify other possible genes in Zhoumai 22, ten P. triticina races avirulent on Zhoumai 22 were used to inoculate 24 F2: 3 lines. The same gene conferred resistance to all ten avirulent races. A total of 1300 simple sequence repeat (SSR) markers and 36 EST markers on 2BS were used to test the parents, and resistant and susceptible bulks. Resistance gene LrZH22 was mapped in the chromosome bin 2BS1-0.53-0.75 and closely linked to six SSR markers (barc183, barc55, gwm148, gwm410, gwm374 and wmc474) and two EST markers (BF202681 and BE499478) on chromosome arm 2BS. The two closest flanking SSR loci were Xbarc55 and Xgwm374 with genetic distances of 2.4 and 4.8 cM from LrZH22, respectively. Six designated genes (Lr13, Lr16, Lr23, Lr35, Lr48 and Lr73) are located on chromosome arm 2BS. In seedling tests, LrZH22 was temperature sensitive, conferring resistance at high temperatures. The reaction pattern of Zhoumai 22 was different from that of RL 4031 (Lr13), RL 6005 (Lr16) and RL 6012 (Lr23), Lr35 and Lr48 are adult-plant resistance genes, and Lr73 is not sensitive to the temperature. Therefore, LrZH22 is likely to be a new leaf rust resistance gene or allele.
学科主题	Horticulture
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000379158900007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105271]
专题	作物科学研究所
作者单位	1.Hebei Agr Univ, Coll Plant Protect, Biol Control Ctr Plant Dis & Plant Pests Hebei, 289 Lingyusi St, Baoding 071001, Hebei, Peoples R China 2.Zhoukou Acad Agr Sci, Jianshe East Rd, Zhoukou 466001, Henan, Peoples R China 3.CAAS, Inst Crop Sci, Natl Wheat Improvement Ctr, 12 Zhongguancun South St, Beijing 100081, Peoples R China 4.CAAS, Int Maize & Wheat Improvement Ctr CIMMYT China Of, 12 Zhongguancun South St, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Cuifen,Yin, Guihong,Xia, Xianchun,et al. Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22[J]. MOLECULAR BREEDING,2016,36(2):-.
APA	Wang, Cuifen.,Yin, Guihong.,Xia, Xianchun.,He, Zhonghu.,Zhang, Peipei.,...&Liu, Daqun.(2016).Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22.MOLECULAR BREEDING,36(2),-.
MLA	Wang, Cuifen,et al."Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22".MOLECULAR BREEDING 36.2(2016):-.