CRISPR/Cas9: A powerful tool for crop genome editing

doi:10.1016/j.cj.2015.12.002

CORC > 中国农业科学院 > 作物科学研究所

	CRISPR/Cas9: A powerful tool for crop genome editing
	Song Gaoyuan; Jia Meiling; Chen Kai; Kong Xingchen; Khattak Bushra; Xie Chuanxiao; Li Aili; Mao Long
刊名	The Crop Journal
	2016
卷号	4 期号:2 页码:75-82
关键词	CRISPR/Cas9 Double-strand break Genome editing TALENs ZFNs
ISSN号	2095-5421
DOI	10.1016/j.cj.2015.12.002
英文摘要	The CRISPR/Cas9 technology is evolved froma type II bacterial immune systemand represents a new generation of targeted genome editing technology that can be applied to nearly all organisms. Site-specific modification is achieved by a single guide RNA (usually about 20 nucleotides) that is complementary to a target gene or locus and is anchored by a protospaceradjacent motif. Cas9 nuclease then cleaves the targeted DNA to generate double-strand breaks (DSBs), which are subsequently repaired by non-homologous end joining (NHEJ) or homology-directed repair (HDR) mechanisms. NHEJ may introduce indels that cause frame shift mutations and hence the disruption of gene functions. When combined with double or multiplex guide RNA design, NHEJ may also introduce targeted chromosome deletions, whereas HDR can be engineered for target gene correction, gene replacement, and gene knock-in. In this review, webriefly survey the history of the CRISPR/Cas9 systeminvention and its genome-editing mechanism. We also describe the most recent innovation of the CRISPR/ Cas9 technology, particularly the broad applications ofmodified Cas9 variants, and discuss the potential of this systemfor targeted genome editing and modification for crop improvement.
学科主题	农业基础科学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101671]
专题	作物科学研究所
作者单位	Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing, 100081
推荐引用方式 GB/T 7714	Song Gaoyuan,Jia Meiling,Chen Kai,et al. CRISPR/Cas9: A powerful tool for crop genome editing[J]. The Crop Journal,2016,4(2):75-82.
APA	Song Gaoyuan.,Jia Meiling.,Chen Kai.,Kong Xingchen.,Khattak Bushra.,...&Mao Long.(2016).CRISPR/Cas9: A powerful tool for crop genome editing.The Crop Journal,4(2),75-82.
MLA	Song Gaoyuan,et al."CRISPR/Cas9: A powerful tool for crop genome editing".The Crop Journal 4.2(2016):75-82.