Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection

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	Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection
	Ding Zai-Song; Zhao Ming; Jing Yu-Xiang; Li Liang-Bi; Kuang Ting-Nin
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2006
卷号	24 期号:3-4 页码:295-303
关键词	Agrobacterium-mediated transformation mannose selection transgenic rice
ISSN号	0735-9640
通讯作者	Ding Zai-Song
英文摘要	A positive selection system was developed for Agrobacterium-mediated transformation of rice that does not use toxic Compounds such as antibiotics or herbicides. The selection system is based on the Escherichia coli phosphomannose isomerase (pmi) gene as a selectable marker and mannose as the selective agent. Only transgenic plants were able to metabolize mannose into a usable Source of carbon, fructose. Selection was achieved using a combination of mannose and sucrose at 10 g/L and 5 g/L, respectively. Transgenic rice plants were produced efficiently in japanica rice variety Zhonghua 8, with transformation frequency of 16.5%, which was slightly lower than that achieved by hygromycin selection.
学科主题	Biochemical Research Methods ; Plant Sciences ; BIOCHEMICAL RESEARCH METHODS ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000202988000002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5723]
专题	作物科学研究所
作者单位	(1)Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China;(2)Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China;(3)Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Ding Zai-Song,Zhao Ming,Jing Yu-Xiang,et al. Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection[J]. PLANT MOLECULAR BIOLOGY REPORTER,2006,24(3-4):295-303.
APA	Ding Zai-Song,Zhao Ming,Jing Yu-Xiang,Li Liang-Bi,&Kuang Ting-Nin.(2006).Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection.PLANT MOLECULAR BIOLOGY REPORTER,24(3-4),295-303.
MLA	Ding Zai-Song,et al."Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection".PLANT MOLECULAR BIOLOGY REPORTER 24.3-4(2006):295-303.