Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays)

doi:10.1080/10425170701292051

CORC > 中国农业科学院 > 作物科学研究所

	Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays)
	Li, Hui-Yong; Wang, Tian-Yu; Shi, Yun-Su; Fu, Jun-Jie; Song, Yan-Chun; Wang, Guo-Ying; Li, Yu
刊名	DNA SEQUENCE
	2007
卷号	18 期号:6 页码:443-458
关键词	zea mays drought stress ears silks gene expression profiling
ISSN号	1042-5179
DOI	10.1080/10425170701292051
通讯作者	Li, Hui-Yong
英文摘要	Maize female organs are sensitive to drought stress, leading to reproductive failure and yield reduction. In the present study gene expression profiles of ears and silks of maize at the flowering stage under drought stress were investigated. From 1920 white positive clones of a forward suppression subtractive hybridization (SSH) library, 1439 available sequences of expression sequence tags ( ESTs) were obtained, resulting in 361 unique ESTs after assembling. Data analysis showed that 218 of the unique ESTs had significant protein homology by BLASTX in UNIPROT database. Totally 99 uniESTs were found in TIGR maize gene indices and nr database by BLASTN, while 44 uniESTs were not found to have homologous nucleic acid sequences and putatively classified as "maize-specific" uniESTs. The 218 cDNAs with significant protein homology were sorted into 13 groups according to the functional categories of the Arabidopsis proteins. Among those genes, the genes associated with the metabolisms were the largest group (account for 27%), and the genes related to protein synthesis, protein fate, transcription, cell cycle and DNA processing accounted for 16, 10, 10 and 9%, respectively. After analysis of macroarray data and real-time quantitative polymerase chain reaction (PCR), it was found that 160 of the 218 homologous protein uniESTs were up-regulated genes in the ears, 129 in the silks, and 125 in both of the tissues. The present work provided a valuable starting point for further elucidation of the roles played by these genes/gene products in drought tolerance in maize.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	INFORMA HEALTHCARE-TAYLOR & FRANCIS
WOS记录号	WOS:000248678300005
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5693]
专题	作物科学研究所
作者单位	(1)Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China;(2)China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China
推荐引用方式 GB/T 7714	Li, Hui-Yong,Wang, Tian-Yu,Shi, Yun-Su,et al. Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays)[J]. DNA SEQUENCE,2007,18(6):443-458.
APA	Li, Hui-Yong.,Wang, Tian-Yu.,Shi, Yun-Su.,Fu, Jun-Jie.,Song, Yan-Chun.,...&Li, Yu.(2007).Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays).DNA SEQUENCE,18(6),443-458.
MLA	Li, Hui-Yong,et al."Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays)".DNA SEQUENCE 18.6(2007):443-458.