Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat

doi:10.1007/s00122-006-0445-5

CORC > 中国农业科学院 > 作物科学研究所

	Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat
	Zhao, X. L.; Xia, X. C.; He, Z. H.; Lei, Z. S.; Appels, R.; Yang, Y.; Sun, Q. X.; Ma, W.
刊名	THEORETICAL AND APPLIED GENETICS
	2007
卷号	114 期号:3 页码:451-460
ISSN号	0040-5752
DOI	10.1007/s00122-006-0445-5
通讯作者	Zhao, X. L.
英文摘要	Low-molecular-weight glutenin subunits (LMW-GS) play an important role in bread and noodle processing quality by influencing the viscoelasticity and extensibility of dough. The objectives of this study were to characterize Glu-D3 subunit coding genes and to develop molecular markers for identifying Glu-D3 gene haplotypes. Gene specific primer sets were designed to amplify eight wheat cultivars containing Glu-D3a, b, c, d and e alleles, defined traditionally by protein electrophoretic mobility. Three novel Glu-D3 DNA sequences, designated as GluD3-4, GluD3-5 and GluD3-6, were amplified from the eight wheat cultivars. GluD3-4 showed three allelic variants or haplotypes at the DNA level in the eight cultivars, which were designated as GluD3-41, GluD3-42 and GluD3-43. Compared with GluD3-42, a single nucleotide polymorphism (SNP) was detected for GluD3-43 in the coding region, resulting in a pseudo-gene with a nonsense mutation at the 119th position of deduced peptide, and a 3-bp insertion was found in the coding region of GluD3-41, leading to a glutamine insertion at the 249th position of its deduced protein. The coding regions for GluD3-5 and GluD3-6 showed no allelic variation in the eight cultivars tested, indicating that they were relatively conservative in common wheat. Based on the 12 allelic variants of three Glu-D3 genes identified in this study and three detected previously, seven STS markers were established to amplify the corresponding gene sequences in wheat cultivars containing five Glu-D3 alleles (a, b, c, d and e). The seven primer sets M2F12/M2R12, M2F2/M2R2, M2F3/M2R3, M3F1/M3R1, M3F2/M3R2, M4F1/M4R1 and M4F3/M4R3 were specific to the allelic variants GluD3-21/22, GluD3-22, GluD3-23, GluD3-31, GluD3-32, GluD3-41 and GluD3-43, respectively, which were validated by amplifying 20 Chinese wheat cultivars containing alleles a, b, c and f based on protein electrophoretic mobility. These markers will be useful to identify the Glu-D3 gene haplotypes in wheat breeding programs.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000243691700006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5686]
专题	作物科学研究所
作者单位	(1)Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China;(2)China Agr Univ, Coll Agron & Biotechnol, Beijing 100094, Peoples R China;(3)Henan Acad Agr Sci, Wheat Res Inst, Zhengzhou 450002, Henan, Peoples R China
推荐引用方式 GB/T 7714	Zhao, X. L.,Xia, X. C.,He, Z. H.,et al. Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat[J]. THEORETICAL AND APPLIED GENETICS,2007,114(3):451-460.
APA	Zhao, X. L..,Xia, X. C..,He, Z. H..,Lei, Z. S..,Appels, R..,...&Ma, W..(2007).Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat.THEORETICAL AND APPLIED GENETICS,114(3),451-460.
MLA	Zhao, X. L.,et al."Novel DNA variations to characterize low molecular weight glutenin Glu-D3 genes and develop STS markers in common wheat".THEORETICAL AND APPLIED GENETICS 114.3(2007):451-460.