Proteomic analysis of royal jelly from three strains of western honeybees (Apis mellifera) | |
Li, Jianke; Wang, Ting; Zhang, Zhaohui; Pan, Yinghong | |
刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
2007 | |
卷号 | 55期号:21页码:8411-8422 |
关键词 | royal jelly (RJ) major royal jelly protein (MRJP) honeybee (Apis mellifera) proteome |
ISSN号 | 0021-8561 |
DOI | 10.1021/jf0717440 |
通讯作者 | Li, Jianke |
英文摘要 | To compare the protein complement of royal jelly (RJ) from high RJ producing honeybees (Apis mellifera L.), a strain of A. mellifera artificially selected for increased RJ production from Italian honeybees in China for more than two decades was compared to those of native Italian honeybees (A. mellifera L.) and Carnica honeybees (A. mellifera C.); the protein in RJ from these three strains of honeybees was partially identified by using a combination of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS), and a protein engine identification tool applied to the honeybee genome. The results showed that 152, 157, and 137 proteins were detected in the three species of RJ; among which 57, 57, 51 high abundant proteins ere identified, respectively. Most identifited spots, 45, 45, 41, were assigned to major royal jelly proteins (MRJPs). Remarkable differences were found in the heterogeneity of the MRJPs, in particular, MRJP3. Also, 3-glucose oxidase, 1-peroxiredoxin (PRDX), and 1-glutathione S-transferase (GST) S1 were identified in three RJ samples. Furthermore, during the determination of the peptides mass fingerprinting (PMF) of each spot, for the first time, PRDX and GST S1 proteins have been identified in RJ. Thus, the results suggest that the protein complement of high RJ producing honeybees is not different compared to native Italian honeybees, while a difference remains between Carnica honeybees. |
学科主题 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology ; AGRICULTURE, MULTIDISCIPLINARY ; CHEMISTRY, APPLIED ; FOOD SCIENCE & TECHNOLOGY |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000250110600018 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/5654] |
专题 | 作物科学研究所 |
作者单位 | (1)Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China;(2)Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Peoples R China;(3)Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet I, Beijing 100081, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Jianke,Wang, Ting,Zhang, Zhaohui,et al. Proteomic analysis of royal jelly from three strains of western honeybees (Apis mellifera)[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2007,55(21):8411-8422. |
APA | Li, Jianke,Wang, Ting,Zhang, Zhaohui,&Pan, Yinghong.(2007).Proteomic analysis of royal jelly from three strains of western honeybees (Apis mellifera).JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,55(21),8411-8422. |
MLA | Li, Jianke,et al."Proteomic analysis of royal jelly from three strains of western honeybees (Apis mellifera)".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 55.21(2007):8411-8422. |
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