Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice

doi:10.1007/s10681-007-9422-6

CORC > 中国农业科学院 > 作物科学研究所

	Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice
	Qiao, Feng; Yang, Qing; Wang, Chun-Lian; Fan, Ying-Lun; Wu, Xue-Feng; Zhao, Kai-Jun
刊名	EUPHYTICA
	2007
卷号	158 期号:1-2 页码:35-45
关键词	GA20ox plant height Rice (Oryza sativa L) RNAi semi-dwarfism
ISSN号	0014-2336
DOI	10.1007/s10681-007-9422-6
通讯作者	Qiao, Feng
英文摘要	GA 20-oxidase (GA20ox) is a regulatory enzyme for the syntheses of biologically active GAs in plants. The loss-of-function mutations in OsGA20ox2 of rice (Oryza sativa L.) generate the well-known Green Revolution gene sd-1, which cause the semi-dwarfism phenotype. In our present investigation, semi-dwarf plants were generated from a taller rice variety QX1 by RNAi suppression on the expression of OsGA20ox2. The 531bp-fragment of OsGA20ox2 was amplified by PCR from genomic DNA of QX1 and used to construct the hairpin RNAi vector pCQK2. The wild type QX1 was transformed with pCQK2 by Agrobacterium-mediated transformation and some independent transgenic RNAi lines exhibited semi-dwarfism. RT-PCR and Northern blot analyses showed that the expression of OsGA20ox2 was specifically suppressed in the RNAi semi-dwarf lines. Endogenous GA assays revealed that the contents of the GA20ox2-catalyzed products GA(19), GA(20) and the down-stream biologically active GA(1) were drastically reduced in the RNAi semi-dwarf lines. We further showed that the RNAi semi-dwarf lines could be restored to normal plant height by applying exogenous GA(3). The results indicated that the semi-dwarfism of the RNAi semi-dwarf lines was associated with the decreased expression of OsGA20ox2 gene and the reduced content of endogenous biologically active GA(1). Analyses of panicle length, seeds per panicle and 1000-grain weight suggested that the RNAi semi-dwarf lines showed stable grain yield compared with the wild type plants. It is demonstrated that the RNAi approach could be useful for plant breeding purposes in the future.
学科主题	Agronomy ; Plant Sciences ; Horticulture ; AGRONOMY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000250061900004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5651]
专题	作物科学研究所
作者单位	(1)Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China;(2)Chinese Acad Agr Sci, Inst Crop Sci, Minist Agr, Key Lab Crop Genet & Breeding, Beijing 100081, Peoples R China;(3)Natl Key Facil, Crop Gene Resources & Genet Improvement, Beijing 100081, Peoples R China;(4)IRRI, China Off, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Feng,Yang, Qing,Wang, Chun-Lian,et al. Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice[J]. EUPHYTICA,2007,158(1-2):35-45.
APA	Qiao, Feng,Yang, Qing,Wang, Chun-Lian,Fan, Ying-Lun,Wu, Xue-Feng,&Zhao, Kai-Jun.(2007).Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice.EUPHYTICA,158(1-2),35-45.
MLA	Qiao, Feng,et al."Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice".EUPHYTICA 158.1-2(2007):35-45.