Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana

doi:10.1007/s00425-009-1092-2

CORC > 中国农业科学院 > 作物科学研究所

	Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana
	Chen, Jie-Yin; Dai, Xiao-Feng
刊名	PLANTA
	2010
卷号	231 期号:4 页码:861-873
关键词	Flavonoid Induce expression Major latex proteins Salt stress Verticillium wilt resistance
ISSN号	0032-0935
DOI	10.1007/s00425-009-1092-2
通讯作者	Chen, Jie-Yin
英文摘要	The major latex protein (MLP) gene in Gossypium hirsutum was cloned and designated Gh-MLP. Expression in cotton root was induced by salt stress and Verticillium dahliae toxin, and bioinformatic analysis showed that Gh-MLP encodes a 157-amino acid protein that is similar to members of the MLP subfamily in the Bet v 1 family. Although the structure of MLP is similar to Bet v 1 family proteins, the sequence identity to other subfamilies of Bet v 1 proteins is less than 20%. The Gh-MLP promoter contains potential cis-acting elements for response to salt stress and fungal elicitor. RT-PCR analysis showed that Gh-MLP expression was rapidly induced by NaCl and V. dahliae toxin, and induction was maintained over 72 h. However, Gh-MLP transgenic Arabidopsis thaliana did not show resistance to V. dahiae, salt tolerance was significantly enhanced. In contrast to the wild type, the Gh-MLP transgene allowed plants to germinate normally after treatment with 75 mM NaCl. Total flavonoid was twofold higher in transgenic Arabidopsis than in the control, suggesting that Gh-MLP might be involved in altering flavonoid content. We hypothesize Gh-MLP, like other Bet v 1 family proteins, participates in the binding or transport of ligands through its specific three-dimensional structure, and takes part in defensive responses to biotic and abiotic stresses.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000274468800007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5441]
专题	作物科学研究所
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jie-Yin,Dai, Xiao-Feng. Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana[J]. PLANTA,2010,231(4):861-873.
APA	Chen, Jie-Yin,&Dai, Xiao-Feng.(2010).Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana.PLANTA,231(4),861-873.
MLA	Chen, Jie-Yin,et al."Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana".PLANTA 231.4(2010):861-873.