Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.)

doi:10.1007/s00425-010-1313-8

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	Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.)
	Zhao, Z. G.1; Zhu, S. S.2; Zhang, Y. H.1; Bian, X. F.1; Wang, Y.1; Jiang, L.1; Liu, X.1; Chen, L. M.1; Liu, S. J.1; Zhang, W. W.1
刊名	PLANTA
	2011
卷号	233 期号:3 页码:485-494
关键词	Chromosome segment substitution lines Fine mapping Hybrid sterility Molecular breeding Proteins for gamete genesis
ISSN号	0032-0935
DOI	10.1007/s00425-010-1313-8
通讯作者	Zhao, Z. G.
英文摘要	Hybrid sterility hinders the exploitation of the heterosis displayed by japonica x indica rice hybrids. The variation in pollen semi-sterility observed among hybrids between the japonica recipient cultivar and each of two sets of chromosome segment substitution lines involving introgression from an indica cultivar was due to a factor on chromosome 5 known to harbor the gene S24. S24 was fine mapped to a 42 kb segment by analyzing a large F(2) population bred from the cross S24-NIL x Asominori, while the semi-sterility shown by the F(1) hybrid was ascribable to mitotic failure at the early bicellular pollen stage. Interestingly, two other pollen sterility genes (f5-Du and Sb) map to the same region (Li et al. in Chin Sci Bull 51:675-680, 2006; Wang et al. in Theor Appl Genet 112:382-387, 2006), allowing a search for candidate genes in the 6.4 kb overlap between the three genes. By sequencing the overlapped fragment in wild rice, indica cultivars and japonica cultivars, a protein ankyrin-3 encoded by the ORF2 was identified as the molecular base for S24. A cultivar Dular was found to have a hybrid-sterility-neutral allele, S24-n, in which an insertion of 30 bp was confirmed. Thus, it was possible to add one more case of molecular bases for the hybrid sterility. No gamete abortion is caused on heterozygous maternal genotype with an impaired sequence from the hybrid-sterility-neutral genotype. This result will be useful in understanding of wide compatibility in rice breeding.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000287666300004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5334]
专题	作物科学研究所
作者单位	1.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing 210095, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China 3.Nihon Univ, Dept Breeding, Fujisawa, Kanagawa 2528510, Japan
推荐引用方式 GB/T 7714	Zhao, Z. G.,Zhu, S. S.,Zhang, Y. H.,et al. Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.)[J]. PLANTA,2011,233(3):485-494.
APA	Zhao, Z. G..,Zhu, S. S..,Zhang, Y. H..,Bian, X. F..,Wang, Y..,...&Wan, J. M..(2011).Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.).PLANTA,233(3),485-494.
MLA	Zhao, Z. G.,et al."Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.)".PLANTA 233.3(2011):485-494.