Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1

doi:10.1007/s00299-011-1129-4

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	Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1
	Bian, X. F.1; Liu, X.1; Zhao, Z. G.1; Jiang, L.1; Gao, H.1; Zhang, Y. H.1; Zheng, M.1; Chen, L. M.1; Liu, S. J.1; Zhai, H. Q.2
刊名	PLANT CELL REPORTS
	2011
卷号	30 期号:12 页码:2243-2254
关键词	Near-isogenic lines Days to heading Flowering regulator African rice MADS-box
ISSN号	0721-7714
DOI	10.1007/s00299-011-1129-4
通讯作者	Bian, X. F.
英文摘要	Heading date in rice is an important agronomic trait controlled by several genes. In this study, flowering time of variety Dianjingyou 1 (DJY1) was earlier than a near-isogenic line (named NIL) carried chromosome segment from African rice on chromosome 3S, when grown in both long-day (LD) and short-day (SD) conditions. By analyzing a large F(2) population from NIL x DJY1, the locus DTH3 (QTL for days to heading on chromosome 3) controlling early heading date in DJY1 was fine mapped to a 64-kb segment which contained only one annotated gene, a MIKC-type MADS-box protein. We detected a 6-bp deletion and a single base substitution in the C-domain by sequencing DTH3 in DJY1 compared with dth3 in NIL, and overexpression of DTH3 caused early flowering in callus. Quantitative real-time PCR revealed that the transcript level of dth3 in NIL was lower than that DTH3 in DJY1 in both LD and SD conditions. The Early heading date 1 (Ehd1) which promotes the RFT1, was up-regulated by DTH3 in both LD and SD conditions. Based on Indel and dCAPs marker analysis, the dth3 allele was only present in African rice accessions. A phylogenetic analysis based on microsatellite genotyping suggested that African rice had a close genetic relationship to O. rufipogon and O. latifolia, and was similar to japonica cultivars. DTH3 affected flowering time and had no significant effect on the main agronomic traits.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000297152400009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5247]
专题	作物科学研究所
作者单位	1.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing 210095, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Bian, X. F.,Liu, X.,Zhao, Z. G.,et al. Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1[J]. PLANT CELL REPORTS,2011,30(12):2243-2254.
APA	Bian, X. F..,Liu, X..,Zhao, Z. G..,Jiang, L..,Gao, H..,...&Wan, J. M..(2011).Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1.PLANT CELL REPORTS,30(12),2243-2254.
MLA	Bian, X. F.,et al."Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1".PLANT CELL REPORTS 30.12(2011):2243-2254.