A PP6-Type Phosphatase Holoenzyme Directly Regulates PIN Phosphorylation and Auxin Efflux in Arabidopsis

doi:10.1105/tpc.112.098905

CORC > 中国农业科学院 > 作物科学研究所

	A PP6-Type Phosphatase Holoenzyme Directly Regulates PIN Phosphorylation and Auxin Efflux in Arabidopsis
	Dai, Mingqiu 1; Zhang, Chen 2,3; Kania, Urszula 4,5; Chen, Fang 1; Xue, Qin 1; Mccray, Tyra 1; Li, Gang 1; Qin, Genji 6; Wakeley, Michelle 1,7; Terzaghi, William 1,7
刊名	PLANT CELL
	2012
卷号	24 期号:6 页码:2497-2514
ISSN号	1040-4651
DOI	10.1105/tpc.112.098905
通讯作者	Dai, Mingqiu
英文摘要	The directional transport of the phytohormone auxin depends on the phosphorylation status and polar localization of PIN-FORMED (PIN) auxin efflux proteins. While PINIOD (PID) kinase is directly involved in the phosphorylation of PIN proteins, the phosphatase holoenzyme complexes that dephosphorylate PIN proteins remain elusive. Here, we demonstrate that mutations simultaneously disrupting the function of Arabidopsis thaliana FyPP1 (for Phytochrome-associated serine/threonine protein phosphatase1) and FyPP3, two homologous genes encoding the catalytic subunits of protein phosphatase6 (PP6), cause elevated accumulation of phosphorylated PIN proteins, correlating with a basal-to-apical shift in subcellular PIN localization. The changes in PIN polarity result in increased root basipetal auxin transport and severe defects, including shorter roots, fewer lateral roots, defective columella cells, root meristem collapse, abnormal cotyledons (small, cup-shaped, or fused cotyledons), and altered leaf venation. Our molecular, biochemical, and genetic data support the notion that FyPP1/3, SAL (for SAPS DOMAIN-LIKE), and PP2AA proteins (RCN1 [for ROOTS CURL IN NAPHTHYLPHTHALAMIC ACID1] or PP2AA1, PP2AA2, and PP2AA3) physically interact to form a novel PP6-type heterotrimeric holoenzyme complex. We also show that FyPP1/3, SAL, and PP2AA interact with a subset of PIN proteins and that for SAL the strength of the interaction depends on the PIN phosphorylation status. Thus, an Arabidopsis PP6-type phosphatase holoenzyme acts antagonistically with PID to direct auxin transport polarity and plant development by directly regulating PIN phosphorylation.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; CELL BIOLOGY ; PLANT SCIENCES
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000306919300021
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5161]
专题	作物科学研究所
作者单位	1.Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA 2.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore 3.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China 4.Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium 5.Univ Ghent, Dept Plant Biotechnol & Genet, B-9052 Ghent, Belgium 6.Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA 7.Wilkes Univ, Dept Biol, Wilkes Barre, PA 18766 USA 8.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 9.Capital Normal Univ, Beijing 100048, Peoples R China 10.Natl Engn Res Ctr Crop Mol Design, Beijing 100085, Peoples R China
推荐引用方式 GB/T 7714	Dai, Mingqiu,Zhang, Chen,Kania, Urszula,et al. A PP6-Type Phosphatase Holoenzyme Directly Regulates PIN Phosphorylation and Auxin Efflux in Arabidopsis[J]. PLANT CELL,2012,24(6):2497-2514.
APA	Dai, Mingqiu.,Zhang, Chen.,Kania, Urszula.,Chen, Fang.,Xue, Qin.,...&Wang, Haiyang.(2012).A PP6-Type Phosphatase Holoenzyme Directly Regulates PIN Phosphorylation and Auxin Efflux in Arabidopsis.PLANT CELL,24(6),2497-2514.
MLA	Dai, Mingqiu,et al."A PP6-Type Phosphatase Holoenzyme Directly Regulates PIN Phosphorylation and Auxin Efflux in Arabidopsis".PLANT CELL 24.6(2012):2497-2514.