The Ca2+-Regulation of the Mitochondrial External NADPH Dehydrogenase in Plants Is Controlled by Cytosolic pH

doi:10.1371/journal.pone.0139224

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	The Ca2+-Regulation of the Mitochondrial External NADPH Dehydrogenase in Plants Is Controlled by Cytosolic pH
	Hao, Meng-Shu 1; Jensen, Anna M.1,2; Boquist, Ann-Sofie 1; Liu, Yun-Jun 1,3; Rasmusson, Allan G.1
刊名	PLOS ONE
	2015
卷号	10 期号:9 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0139224
通讯作者	Hao, Meng-Shu
英文摘要	NADPH is a key reductant carrier that maintains internal redox and antioxidant status, and that links biosynthetic, catabolic and signalling pathways. Plants have a mitochondrial external NADPH oxidation pathway, which depends on Ca2+ and pH in vitro, but concentrations of Ca2+ needed are not known. We have determined the K-0.5(Ca2+) of the external NADPH dehydrogenase from Solanum tuberosum mitochondria and membranes of E. coli expressing Arabidopsis thaliana NDB1 over the physiological pH range using O-2 and decylubiquinone as electron acceptors. The K-0.5(Ca2+) of NADPH oxidation was generally higher than for NADH oxidation, and unlike the latter, it depended on pH. At pH 7.5, K-0.5(Ca2+) for NADPH oxidation was high (approximate to 100 mu M), yet 20-fold lower K-0.5(Ca2+) values were determined at pH 6.8. Lower K-0.5(Ca2+) values were observed with decylubiquinone than with O-2 as terminal electron acceptor. NADPH oxidation responded to changes in Ca2+ concentrations more rapidly than NADH oxidation did. Thus, cytosolic acidification is an important activator of external NADPH oxidation, by decreasing the Ca2+-requirements for NDB1. The results are discussed in relation to the present knowledge on how whole cell NADPH redox homeostasis is affected in plants modified for the NDB1 gene.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000362170700050
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4658]
专题	作物科学研究所
作者单位	1.Lund Univ, Dept Biol, Lund, Sweden 2.Linnaeus Univ, Dept Forestry & Wood Technol, Vaxjo, Sweden 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Hao, Meng-Shu,Jensen, Anna M.,Boquist, Ann-Sofie,et al. The Ca2+-Regulation of the Mitochondrial External NADPH Dehydrogenase in Plants Is Controlled by Cytosolic pH[J]. PLOS ONE,2015,10(9):-.
APA	Hao, Meng-Shu,Jensen, Anna M.,Boquist, Ann-Sofie,Liu, Yun-Jun,&Rasmusson, Allan G..(2015).The Ca2+-Regulation of the Mitochondrial External NADPH Dehydrogenase in Plants Is Controlled by Cytosolic pH.PLOS ONE,10(9),-.
MLA	Hao, Meng-Shu,et al."The Ca2+-Regulation of the Mitochondrial External NADPH Dehydrogenase in Plants Is Controlled by Cytosolic pH".PLOS ONE 10.9(2015):-.