Genetic Dissection of Leaf-related Traits using 156 Chromosomal Segment Substitution Lines

doi:10.1007/s12374-015-0402-4

CORC > 中国农业科学院 > 作物科学研究所

	Genetic Dissection of Leaf-related Traits using 156 Chromosomal Segment Substitution Lines
	Liu, Xi 1; Liu, Linglong 1; Xiao, Yinhui 1; Liu, Shijia 1; Tian, Yunlu 1; Chen, Liangming 1; Wang, Zhiquan 1; Jiang, Ling 1; Zhao, Zhigang 1; Wan, Jianmin 1,2
刊名	JOURNAL OF PLANT BIOLOGY
	2015
卷号	58 期号:6 页码:402-410
关键词	Chlorophyll content Chromosome segment substitution line (CSSL) Leaf length Leaf width Quantitative trait loci (QTL) Rice (Oryza sativa L.)
ISSN号	1226-9239
DOI	10.1007/s12374-015-0402-4
通讯作者	Liu, Xi
英文摘要	A two-line super-hybrid rice (Oryza sativa L.) variety [Liangyoupei9 (LYP9)] demonstrated superiority over its both parents, viz. elite inbred lines 93-11 and Pei-ai64S (PA64S), as well as other conventional hybrids, and had long been exploited in China. However, the genetic basis of its leaf-related traits, supposed to be an important component for yield potential, remains elusive. Here, initially a set of chromosome segment substitution lines (CSSLs) was constructed, in which the genome of Pei-ai64S has been introgressed into the background of 93-11. This set was developed by marker aided selection, based on 123 polymorphic SSR markers. The introgressed chromosomal segments presented in the 156 CSSLs covered 96.46% of Pei-ai64S genome. Afterwards, the CSSLs were deployed to assess the genetic basis of leaf size (length and width) and chlorophyll content of top three leaves across five different environments. The CSSLs showed transgressive segregation for all of the traits, and significant correlations were detected among most of the traits. A total of 27 quantitative trait loci (QTL) were identified on ten chromosomes, and three QTL cluster affecting related traits were found on chromosome 3, 6, and 8, respectively. Remarkably, two key QTLs, qALW3-1 and qALW3-2, both controlling the antepenultimate leaf width, were identified in all five environments, and their effect were further validated by CSSLs harboring the two QTL alleles. Our results indicate that developing CSSLs is a powerful tool for genetic dissection of quantitative traits. Meanwhile, the QTLs controlling leaf-related traits uncovered here provide useful information for marker-assisted selection in improving the performance of leaf morphology and photosynthetic ability.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000368442200007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4624]
专题	作物科学研究所
作者单位	1.Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xi,Liu, Linglong,Xiao, Yinhui,et al. Genetic Dissection of Leaf-related Traits using 156 Chromosomal Segment Substitution Lines[J]. JOURNAL OF PLANT BIOLOGY,2015,58(6):402-410.
APA	Liu, Xi.,Liu, Linglong.,Xiao, Yinhui.,Liu, Shijia.,Tian, Yunlu.,...&Wan, Jianmin.(2015).Genetic Dissection of Leaf-related Traits using 156 Chromosomal Segment Substitution Lines.JOURNAL OF PLANT BIOLOGY,58(6),402-410.
MLA	Liu, Xi,et al."Genetic Dissection of Leaf-related Traits using 156 Chromosomal Segment Substitution Lines".JOURNAL OF PLANT BIOLOGY 58.6(2015):402-410.