Evaluation of DNA barcode candidates for the discrimination of Artemisia L.

CORC > 新疆理化技术研究所 > 中国科学院新疆理化技术研究所 > 省部共建新疆特有药用资源利用重点实验室

	Evaluation of DNA barcode candidates for the discrimination of Artemisia L.
	Liu, Geyu; Ning, Huixia; Ayidaerhan, Nurbolati; Aisa, Haji Akber
刊名	Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis
	2016
期号	9
关键词	Artemisia DNA barcoding identification variation
英文摘要	Because of the very similar morphologies and wide diversity of Artemisia L. varieties, they are difficult to identify, and there have been many arguments about the systematic classification Artemisia L., especially concerning the division of species. DNA barcode technology is used to rapidly identify species based on standard short DNA sequences. To evaluate seven candidate DNA barcodes (ITS, ITS2, psbA-trnH, rbcL, matK, rpoB, and rpoC1) regarding their ability to identify closely related species of the Artemisia genus in Xinjiang. The corresponding PCR amplification efficiency, detectable genetic divergence, identification efficiency and phylogenetic tree were assessed. We found that the internal transcribed spacer (ITS) region exhibited the highest interspecific divergence, which was significantly higher than the observed intraspecific variation and showed the highest identification efficiency, followed by ITS2, psbA-trnH and, finally, rpoB. matK, rbcL, and rpoC1 performed poorly in this evaluation. ITS, ITS2, and psbA-trnH were able to perfectly identify the tested species Artemisia annua, A. absinthium, A. rupestris, A. tonurnefortiana, A. austriaca, A. dracunculus, A. vulgaris, and A. macrocephala. Therefore, we propose the ITS, ITS2, and psbA-trnH regions as promising DNA barcodes for the closely related species of Artemisia L. in Xinjiang.
收录类别	SCI
内容类型	期刊论文
源URL	[http://ir.xjipc.cas.cn/handle/365002/4839]
专题	新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
作者单位	a Key Laboratory of Plant Resources and Chemistry in Arid Zone , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi , Xinjiang , PR China.
推荐引用方式 GB/T 7714	Liu, Geyu,Ning, Huixia,Ayidaerhan, Nurbolati,et al. Evaluation of DNA barcode candidates for the discrimination of Artemisia L.[J]. Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis,2016(9).
APA	Liu, Geyu,Ning, Huixia,Ayidaerhan, Nurbolati,&Aisa, Haji Akber.(2016).Evaluation of DNA barcode candidates for the discrimination of Artemisia L..Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis(9).
MLA	Liu, Geyu,et al."Evaluation of DNA barcode candidates for the discrimination of Artemisia L.".Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis .9(2016).