Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 植物生理生态研究组

	Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi
	Zhang, Jiao-Lin
刊名	PHOTOSYNTHESIS RESEARCH
	2017
卷号	132 期号:3 页码:293-303
关键词	Chlorophyll fluorescence Methyl viologen P700 oxidation Photosystem I Photoinhibition Superoxide
中文摘要	Our previous studies indicated that high light induced significant photoinhibition of photosystem I (PSI) in the shade-establishing tree species Psychotria henryi. However, the underlying mechanism has not been fully clarified. In the present study, in order to investigate the mechanism of PSI photoinhibition in P. henryi, we treated detached leaves with constant high light in the presence of methyl viologen (MV) or a soluble alpha-tocopherol analog, 2,2,5,7,8-pentamethyl-6-chromanol (PMC). We found that MV significantly depressed photochemical quantum yields in PSI and PSII when compared to PMC. On condition that no PSI photoinhibition happened, although cyclic electron flow (CEF) was abolished in the MV-treated samples, P700 oxidation ratio was maintain at higher levels than the PMC-treated samples. In the presence of PMC, PSI photoinhibition little changed but PSII photoinhibition was significantly alleviated. Importantly, PSI photoinhibition was largely accelerated in the presence of MV, which stimulates the production of superoxide and subsequently other reactive oxygen species at the chloroplast stroma by accepting electrons from PSI. Furthermore, MV largely aggravated PSII photoinhibition when compared to control. These results suggest that high P700 oxidation ratio cannot prevent PSI photoinhibition in P. henryi. Furthermore, the superoxide produced in the chloroplast stroma is critical for PSI photoinhibition in the higher plant P. henryi, which is opposite to the mechanism underlying PSI photoinhibition in Arabidopsis thaliana and spinach. These findings highlight a new mechanism of PSI photoinhibition in higher plants.
语种	英语
公开日期	2017-07-18
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/10499]
专题	西双版纳热带植物园_植物生理生态研究组
推荐引用方式 GB/T 7714	Zhang, Jiao-Lin. Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi[J]. PHOTOSYNTHESIS RESEARCH,2017,132(3):293-303.
APA	Zhang, Jiao-Lin.(2017).Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi.PHOTOSYNTHESIS RESEARCH,132(3),293-303.
MLA	Zhang, Jiao-Lin."Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi".PHOTOSYNTHESIS RESEARCH 132.3(2017):293-303.