Affinity and Specificity of Levamlodipine-Human Serum Albumin Interactions: Insights into Its Carrier Function

	Affinity and Specificity of Levamlodipine-Human Serum Albumin Interactions: Insights into Its Carrier Function
	Liu ZJ ; Zheng XL ; Yang XR ; Wang EK ; Wang J
刊名	biophysical journal
	2009
卷号	96 期号:10 页码:3917-3925
关键词	LIGAND-BINDING FLUORESCENCE TRYPTOPHAN RESIDUES REVEALS SYSTEM OXYGEN PROBE
ISSN号	0006-3495
通讯作者	yang xr
中文摘要	the affinity and specificity of drugs with human serum albumin (hsa) are crucial factors influencing the bioactivity of drugs. to gain insight into the carrier function of hsa, the binding of levamlodipine with hsa has been investigated as a model system by a combined experimental and theoretical/computational approach. the fluorescence properties of hsa and the binding parameters of levamlodipine indicate that the binding is characterized by one binding site with static quenching mechanism, which is related to the energy transfer. as indicated by the thermodynamic analysis, hydrophobic interaction is the predominant force in levamiodipine-hsa complex, which is in agreement with the computational results. and the hydrogen bonds can be confirmed by computational approach between levamlodipine and hsa. compared to predicted binding energies and binding energy spectra at seven sites on hsa, levamlodipine binding hsa at site i has a high affinity regime and the highest specificity characterized by the largest intrinsic specificity ratio (isr). the binding characteristics at site i guarantee that drugs can be carried and released from hsa to carry out their specific bioactivity.
收录类别	SCI
语种	英语
WOS记录号	WOS:000266312900005
公开日期	2010-05-27
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/12203]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Liu ZJ,Zheng XL,Yang XR,et al. Affinity and Specificity of Levamlodipine-Human Serum Albumin Interactions: Insights into Its Carrier Function[J]. biophysical journal,2009,96(10):3917-3925.
APA	Liu ZJ,Zheng XL,Yang XR,Wang EK,&Wang J.(2009).Affinity and Specificity of Levamlodipine-Human Serum Albumin Interactions: Insights into Its Carrier Function.biophysical journal,96(10),3917-3925.
MLA	Liu ZJ,et al."Affinity and Specificity of Levamlodipine-Human Serum Albumin Interactions: Insights into Its Carrier Function".biophysical journal 96.10(2009):3917-3925.