Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 条件保障部信息与网络中心

	Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation
	Zhang, Xiaomei 1; Hua, Xiufu 2; Yue, Xiuping 3
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2017-02-01
卷号	101 期号:4 页码:1697-1707
关键词	Quinoline degradation Shortcut denitrification Bacterial community PCR-DGGE High-throughput sequencing qPCR
英文摘要	For quinoline-denitrifying degradation, very few researches focused on shortcut denitrification process and its bacterial community characteristics. In this study, complete and shortcut denitrification systems were constructed simultaneously for quinoline degradation. By calculation, specific quinoline removal rates were 0.905 and 1.123 g/(gVSS d), respectively, in the complete and shortcut systems, and the latter was 1.24 times of the former. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), high-throughput sequencing, and quantitative PCR (qPCR) techniques based on 16S rRNA were jointly applied to compare microbial community structures of two systems. Many denitrifying bacteria phyla, classes, and genera were detected in the two systems. Phylum Proteobacteria, Class Gammaproteobacteria, and Genus Alicycliphilus denitrificans were the dominant contributors for quinoline-denitrifying degradation. In the shortcut denitrification system, main and specific strains playing crucial roles were more; the species richness and the total abundance of functional genes (narG, nirS, nirK, and nosZ) were higher compared with the complete denitrification system. It could be supposed that inorganic-nitrogen reductase activity of bacterial community was stronger in the shortcut denitrification system, which was the intrinsic reason to result in higher denitrification rate.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	RIBOSOMAL-RNA GENE ; WASTE-WATER ; MICROBIAL COMMUNITY ; FUNCTIONAL GENES ; HYDROGENOTROPHIC DENITRIFICATION ; CONSTRUCTED WETLAND ; DIVERSITY ; REACTOR ; POPULATIONS ; PCR
收录类别	SCI
语种	英语
WOS记录号	WOS:000393687400033
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9321]
专题	青岛生物能源与过程研究所_规划战略与信心中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Tsinghua Univ, Dept Sci Res & Dev, Beijing 100084, Peoples R China 3.Taiyuan Univ Technol, Environm Sci & Engn Inst, 79 Yingze West St, Taiyuan 030024, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaomei,Hua, Xiufu,Yue, Xiuping. Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(4):1697-1707.
APA	Zhang, Xiaomei,Hua, Xiufu,&Yue, Xiuping.(2017).Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,101(4),1697-1707.
MLA	Zhang, Xiaomei,et al."Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.4(2017):1697-1707.