Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection

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	Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection
	Zheng, Laibao1,2 ; Wan, Yi 1; Qi, Peng1 ; Sun, Yan 1; Zhang, Dun 1; Yu, Liangmin 3,4
刊名	TALANTA
	2017-05-15
卷号	167 页码:600-606
关键词	3D nano-interface ZnO-NR arrays Lectin functionalization Bacterial detection
英文摘要	The detection of pathogenic bacteria is essential in various fields, such as food safety, water environmental analysis, or clinical diagnosis. Although rapid and selective techniques have been achieved based on the fast and specific binding of recognitions elements and target, the sensitive detection of bacterial pathogens was limited by their low targets-binding efficiency. The three-dimensional (3D) nano-biointerface, compared with the two-dimensional (2D) flat substrate, has a much higher binding capacity, which can offer more reactive sites to bind with bacterial targets, resulting in a great improvement of detection sensitivity. Herein, a lectin functionalized ZnO nanorod (ZnO-NR) array has been fabricated and employed as a 3D nano-biointerface for Escherichia coli (E. con) capture and detection by multivalent binding of concanavalin A (ConA) with polysaccharides on the cellular surface of E. coli. The 3D lectin functionalized ZnO-NR array-based assay shows reasonable detection limit and efficiently expanded linear range (1.0x10(3) to 1.0x10(7) cfu mL(-1)) for pathogen detection. The platform has a potential for further applications and provides an excellent sensitivity approach for detection of pathogenic bacteria.
收录类别	SCI
语种	英语
WOS记录号	WOS:000399511600075
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/136699]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Ocean Univ China, Key Lab Marine Chem Theory & Technol, Minist Educ, Qingdao 266100, Peoples R China 4.Ocean Univ China, Qingdao Collaborat Innovat Ctr Marine Sci & Techn, Qingdao 266100, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Laibao,Wan, Yi,Qi, Peng,et al. Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection[J]. TALANTA,2017,167:600-606.
APA	Zheng, Laibao,Wan, Yi,Qi, Peng,Sun, Yan,Zhang, Dun,&Yu, Liangmin.(2017).Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection.TALANTA,167,600-606.
MLA	Zheng, Laibao,et al."Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection".TALANTA 167(2017):600-606.