Effects of Polyphyllin D, a steroidal saponin in Paris polyphylla, in growth inhibition of human breast cancer cells and in xenograft

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	Effects of Polyphyllin D, a steroidal saponin in Paris polyphylla, in growth inhibition of human breast cancer cells and in xenograft
	Mei-Sze Lee ; Judy Chan Yuet-Wa ; Yu B(俞飚) ; Vincent Ooi Eng-Choon ; Henry Wong Nai-Ching ; Thomas Mak Chung-Wai ; Kwok-Pui Fung
刊名	Cancer Biol. Ther.
	2005
卷号	4 期号:11 页码:1248-1254
ISSN号	1538-4047
其他题名	Effects of Polyphyllin D, a steroidal saponin in Paris polyphylla, in growth inhibition of human breast cancer cells and in xenograft
通讯作者	Kwok-Pui Fung
英文摘要	Paris Polyphylla is a traditional Chinese Medical herb that has been used in treating cancer for thousands of year. Without studies on the anticancer effects of Paris Polyphylla being initiated before, we have first studied the component of Paris Polyphylla and have spotted out a steroidal saponin, polyphyllin D. As long as the chemical structure and the improved synthesis of polyphyllin D were ascertained, both in vitro to in vivo studies were performed. It was found that treatment of MCF-7 and MDA-MB-231 cells with polyphyllin D resulted in the inhibition of viability and induction of apoptosis in a dose-dependent manner, with an IC50 of 5?M and 2.5?M, respectively, after 48 hours of incubations. Apoptosis of MCF-7 and MDA-MB-231 cells by polyphyllin D was evidenced by the occurrence of DNA fragmentation, formation of a hypodiploid peak in the cell cycle analysis, phosphatidyl-serine externalization and a late loss of membrane integrity. Mechanistically, polyphyllin D dissipates the mitochondrial membrane potential, induces a down-regulation of anti-apoptotic Bcl-2 expression and an up-regulation of pro-apoptotic Bax expression, and activates caspase-9. These results suggest that polyphyllin D elicits apoptosis through mitochondria dysfunction. In vivo study demonstrated that daily administration of polyphyllin D (2.73 mg/kg body weight) through intravenous injection for ten days in nude mice bearing MCF-7 cells effectively reduced tumor growth for 50% in terms of tumor weight and size, given no significant toxicity in heart and liver to the host. All these findings provide novel insights that polyphyllin D could serve as a candidate in breast cancer treatment.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.4161/cbt.4.11.2136
语种	英语
WOS记录号	WOS:000236044100021
公开日期	2013-02-25
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/19029]
专题	上海有机化学研究所_生命有机化学国家重点实验室
推荐引用方式 GB/T 7714	Mei-Sze Lee,Judy Chan Yuet-Wa,Yu B,et al. Effects of Polyphyllin D, a steroidal saponin in Paris polyphylla, in growth inhibition of human breast cancer cells and in xenograft[J]. Cancer Biol. Ther.,2005,4(11):1248-1254.
APA	Mei-Sze Lee.,Judy Chan Yuet-Wa.,俞飚.,Vincent Ooi Eng-Choon.,Henry Wong Nai-Ching.,...&Kwok-Pui Fung.(2005).Effects of Polyphyllin D, a steroidal saponin in Paris polyphylla, in growth inhibition of human breast cancer cells and in xenograft.Cancer Biol. Ther.,4(11),1248-1254.
MLA	Mei-Sze Lee,et al."Effects of Polyphyllin D, a steroidal saponin in Paris polyphylla, in growth inhibition of human breast cancer cells and in xenograft".Cancer Biol. Ther. 4.11(2005):1248-1254.