Adenine nucleotide N-Glycosidase activity of the A-chain of chinnamomin characterized by ~1Hnuclear magnetic resonance

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	Adenine nucleotide N-Glycosidase activity of the A-chain of chinnamomin characterized by ~1Hnuclear magnetic resonance
	Xu YZ(徐永镇) ; Li YJ(李泳江) ; Hu HY(胡红雨) ; Wu HM(吴厚铭) ; Liu WY(刘望夷)
刊名	Biol. Chem.
	2000
卷号	381 期号:5-6 页码:447-451
ISSN号	1431-6730
其他题名	香樟子蛋白A链的腺嘌啉核苷N-糖苷酶活性的核磁共振表征
通讯作者	刘望夷
英文摘要	plantribosome-inactivating proteins specifically cleave an N-glyoosidic bond of a unique adenosine in The largest ribosomal RNA, releasing an adenine from ribosomes of differentsources. Here, ~1H-nuclear magnetic resonance is useed to analyze the enzymatic products of the A-chain of cinnamomin. A type-II ribosome-inactvating protein (RIP) actng on the nucleotides insitu. The enzymatic of the RIP on nine nuclootides are compared. Cinnamomin A-chain can cleave the N-glycosidic bond and release an adenine base from adenine nucleotides except 5'-ATP; however it cannot act on 5'-GMP, 5'-CMP, and 5' -UMP The A-chain in the mixture of cinnamomin A- and B-chain exhibits htgher activity toward adenine nucleotides than the A-chain alone does, suggesting that the B-chain canformationally stabilize the A-chain. Intact cinnamomin also exhibits lower activity toward adenine nucleotide, However, cinnamomin B-chain and heat-denatured intact cinnamomin cannot hydrolyze all the tested nucleotides. We conclude That hydrolysis of the N-C glycosidic bond of nucleotide compounds by cinnamomin A-chain has a base preference. And the negatively charged phosphate group(s) reduces the recognition ability of the A-chain to adenine nucleotide.
学科主题	天然产物有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1515/BC.2000.058
语种	英语
公开日期	2013-02-21
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/17267]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	Xu YZ,Li YJ,Hu HY,et al. Adenine nucleotide N-Glycosidase activity of the A-chain of chinnamomin characterized by ~1Hnuclear magnetic resonance[J]. Biol. Chem.,2000,381(5-6):447-451.
APA	徐永镇,李泳江,胡红雨,吴厚铭,&刘望夷.(2000).Adenine nucleotide N-Glycosidase activity of the A-chain of chinnamomin characterized by ~1Hnuclear magnetic resonance.Biol. Chem.,381(5-6),447-451.
MLA	徐永镇,et al."Adenine nucleotide N-Glycosidase activity of the A-chain of chinnamomin characterized by ~1Hnuclear magnetic resonance".Biol. Chem. 381.5-6(2000):447-451.