Nanostructured interfaces with site-specific bioreceptors for immunosensing

	Nanostructured interfaces with site-specific bioreceptors for immunosensing
	Paiva TO; Almeida I; Marques JT; Liu W; Niu Y(牛宇); Jin G(靳刚); Viana AS
刊名	APPLIED SURFACE SCIENCE
	2017-08-01
卷号	412 页码:455-463
关键词	Gold surface modification Protein A nanobioconjugates In situ dithiocarbamate formation Inhibition of nonspecific adsorption Antibody oriented immobilization Total internal reflection imaging ellipsometry
ISSN号	0169-4332
中文摘要	In this work we propose a simple and effective approach to build nanostructured immunosensor platforms. The one-step strategy relies on i) the in situ formation of dithiocarbamates from the reaction between carbon disulfide and amine groups present on protein A ii) their attachment to gold nanopartides (AuNPs) and iii) the linkage of the modified AuNPs to the electrode surface which depends on the strong interaction between gold substrates and sulfur moieties. AuNPs and protein A are used to increase the surface coverage of Immunoglobulin G (IgG) and promote the oriented immobilization of the antibodies on the immunosensing interface. The modified gold surfaces with biomolecules were thoroughly characterized by a combination of techniques: UV-vis spectrophotometry conventional ellipsometry and atomic force microscopy. The immunosensor performance was assessed in real-time by surface plasmon resonance and by the highly sensitive total internal reflection imaging ellipsometry through the specific biorecognition between anti-IgG and the immobilized IgG molecules. We demonstrate that the presence of AuNPs improves the sensitivity of the anti-IgG specific detection whereas the presence of co-adsorbed CS2 is responsible for blocking the undesired protein nonspecific adsorption to the gold substrate. Overall we report a simple and innovative one-step method to chemically modify gold surfaces with protein A and AuNPs able to specifically detect antigen antibody interactions with capability of preventing protein nonspecific adsorption. (C) 2017 Elsevier B.V. All rights reserved.
类目[WOS]	Chemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter
研究领域[WOS]	Chemistry ; Materials Science ; Physics
关键词[WOS]	SELF-ASSEMBLED MONOLAYERS ; SURFACE-PLASMON RESONANCE ; INTERNAL-REFLECTION ELLIPSOMETRY ; NANOPARTICLE THIN-FILMS ; GOLD NANOPARTICLES ; ORIENTED IMMOBILIZATION ; IMAGING ELLIPSOMETRY ; CARBON-DISULFIDE ; SERUM SAMPLES ; PROTEIN
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000401392900051
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/60526]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Paiva TO,Almeida I,Marques JT,et al. Nanostructured interfaces with site-specific bioreceptors for immunosensing[J]. APPLIED SURFACE SCIENCE,2017,412:455-463.
APA	Paiva TO.,Almeida I.,Marques JT.,Liu W.,牛宇.,...&Viana AS.(2017).Nanostructured interfaces with site-specific bioreceptors for immunosensing.APPLIED SURFACE SCIENCE,412,455-463.
MLA	Paiva TO,et al."Nanostructured interfaces with site-specific bioreceptors for immunosensing".APPLIED SURFACE SCIENCE 412(2017):455-463.