Comparison of medium-chain-length polyhydroxyalkanoates synthases from Pseudomonas mendocina NK-01 with the same substrate specificity

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	Comparison of medium-chain-length polyhydroxyalkanoates synthases from Pseudomonas mendocina NK-01 with the same substrate specificity
	Guo, Wenbi ; Duan, Jingjing ; Geng, Weit ; Feng, J ; Wang, Shufa ; Song, Cunjia ; Duan JJ(段静静)
刊名	http://dx.doi.org/10.1016/j.micres.2012.11.003
	2013-05-06
关键词	Bacteria Chromatography Cloning Differential scanning calorimetry Gel permeation chromatography Gene expression Thermogravimetric analysis X ray diffraction
英文摘要	The medium-chain-length polyhydroxyalkanoate (PHAMCL) synthase genes phaC1 and phaC2 of Pseudomonas mendocina NK-01 were cloned and inserted into expression plasmid pBBR1MCS-2 to form pBBR1MCS-C1 and pBBR1MCS-C2 which were expressed respectively in the PHAMCL-negative strain P. mendocina C7 whose PHAMCL synthesis operon was defined knock out. P. mendocina C7 derivatives P. mendocina C7C1 and C7C2 carrying pBBR1MCS-C1 and pBBR1MCS-C2 respectively were constructed. Fermentation and gel permeation chromatography (GPC) revealed that P. mendocina C7C1 had higher PHAMCL production rate but its PHAMCL had lower molecular weight than that of P. mendocina C7C2. Gas chromatograph/mass spectrometry (GC/MS) analysis revealed that the two PHAMCL had similarity in monomer composition with 3HD as the favorite monomer i.e. PhaC1 and PhaC2 had the same substrate specificity. Differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and X-ray diffraction (XRD) also revealed that the two PHAMCL had the same physical properties. P. mendocina NK-01was the first reported strain whose PHAMCL synthases PhaC1 and PhaC2 had the same substrate specificity. ? 2012 Elsevier GmbH.
语种	英语
出版者	Urban und Fischer Verlag Jena
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89932]
专题	环境生态－已发表论文
推荐引用方式 GB/T 7714	Guo, Wenbi,Duan, Jingjing,Geng, Weit,et al. Comparison of medium-chain-length polyhydroxyalkanoates synthases from Pseudomonas mendocina NK-01 with the same substrate specificity[J]. http://dx.doi.org/10.1016/j.micres.2012.11.003,2013.
APA	Guo, Wenbi.,Duan, Jingjing.,Geng, Weit.,Feng, J.,Wang, Shufa.,...&段静静.(2013).Comparison of medium-chain-length polyhydroxyalkanoates synthases from Pseudomonas mendocina NK-01 with the same substrate specificity.http://dx.doi.org/10.1016/j.micres.2012.11.003.
MLA	Guo, Wenbi,et al."Comparison of medium-chain-length polyhydroxyalkanoates synthases from Pseudomonas mendocina NK-01 with the same substrate specificity".http://dx.doi.org/10.1016/j.micres.2012.11.003 (2013).