| 白子草多糖的分离、纯化及初步分析; Isolation,purification and identification of polysaccharides from Gynura divaricata | |
| 王忠震 ; 张钱钱 ; 昊霞 ; 林兵 ; 刘志宏 ; 宋洪涛 ; 陈磊 | |
| 2015-04-15 | |
| 关键词 | 白子草 白背三七 多糖 提纯 分离 结构分析 Gynura divaricata polysaccharides purification monosaccharide composition |
| 英文摘要 | 对白子草中的多糖进行分离纯化,并对得到的多糖组分的理化性质进行分析。将脱脂后的白子草药材经热水提取,乙醇沉淀,木瓜蛋白酶-SEVAg法去蛋白后,再经dEAE-SEPHArOSE fAST flOW离子交换柱色谱分离,透析除盐纯化得白子草多糖,采用高效液相色谱法,红外光谱和~1H-nMr对其进行组成研究和分析。白子草多糖中的gdPS-2的纯度为87.3%,相对分子质量为2.03x10~4 dA;gdPS-3的纯度为90.9%,相对分子质量为4.29x10~4dA。红外光谱无法确定gdPS-2是否有α构型的呋喃糖苷,但提示gdPS-3可能有α构型的呋喃糖苷键,~1H-nMr分析初步判断gdPS-2和gdPS-3的糖苷键连接方式均为α型。gdPS-2由葡萄糖醛酸和木糖组成,摩尔比为1.1:0.63;gdPS-3由鼠李糖、葡萄糖醛酸、半乳糖、木糖和半乳糖醛酸组成,摩尔比为0.32:6.0:0.21:1.75:4.3。; The purpose of this study was to isolate and purify polysaccharide from Gynuia divancaia and analyze its monosaccharide composition.A water-soluble crude polysaccharide was obtained by hot water extraction,ethanol precipitation and deproteinization after degreasing.The crude polysaccharide then purified with DEAE-Sepharose Fast Flow column chromatography and dialysis.The monosaccharide composition and structure were analyzed by HPLC,UV spectrophotometer and H-NMR.The results showed that the purity and molecular weight of GDPS-2 and GDPS-3 were 87.3%,2.03×10~4 Da and 90.9%,4.29×10~4 Da,respectively.The UV spectrophotometer and ~1H-NMR data suggested that glycosidic bond of GDPS-2 and GDPS-3 were α type.Both GDPs-2 and GDPs-3 were homogeneous polysaccharides,and GDPs-2 was mainly composed of glucuronic acid and xylose at a molar ratio of 1.1:0.63.GDPs-3 was mainly composed of rhamnose,glucuronic acid,galactose,xylose and galacturonic acid at a molar ratio of 0.32:6.0:0.21:1.75:4.3.; 福建省自然科学基金项目(2012J01400) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/123537]  |
| 专题 | 药学院－已发表论文 |
| 推荐引用方式 GB/T 7714 | 王忠震,张钱钱,昊霞,等. 白子草多糖的分离、纯化及初步分析, Isolation,purification and identification of polysaccharides from Gynura divaricata[J],2015. |
| APA | 王忠震.,张钱钱.,昊霞.,林兵.,刘志宏.,...&陈磊.(2015).白子草多糖的分离、纯化及初步分析.. |
| MLA | 王忠震,et al."白子草多糖的分离、纯化及初步分析".(2015). |
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