| 癌症标记蛋白AGR2的原核表达及纯化; Prokaryotic Expression and Purification of Cancer Maker Protein AGR2 | |
| 刘文杰 ; 魏龙刚 ; 陶进 ; 吕忠显 | |
| 2015-10-20 | |
| 关键词 | 前梯度蛋白2 原核表达 分离纯化 AGR2 prokaryotic expression protein purification |
| 英文摘要 | 前梯度蛋白2(AnTErIOr grAdIEnT 2,Agr2)是一种分泌蛋白,在多种腺癌中过表达,是一个潜在的肿瘤诊断和预后判断的标志物。用质粒PgEX4T-1构建Agr2蛋白原核表达载体(PgEX4T-1-△SPAgr2),IPTg诱导表达,考马斯亮蓝染色和WESTErn blOT验证纯化的Agr2蛋白。结果表明,利用大肠埃希菌培养,原核表达载体(PgEX4T-1-△SP-Agr2)在25℃、0.1MMOl/l IPTg诱导16H后Agr2蛋白大量表达,分离纯化得到具有生物活性的成熟Agr2蛋白,这对于开展Agr2生物机制的研究,以及开发Agr2的诊断和治疗试剂具有重要的意义。; Anterior gradient-2(AGR2) is a secreted protein and overexpressed in many tumor tissues of glands.Thus,AGR2 is considered to be a promising biomarker for early diagnosis and prognosis on cancer.It was used to express AGR2 by prokaryotic expresion vector pGEX4T-l-ASP-AGR2 in E.coli.After induction with IPIG,the protein was assessed with Coomassie bright blue gel and Western blot.The results showed that the AGR2 vector was induced with IPTG at 25℃ for 16 hours to get high expression of pure and bioactive AGR2 protein.Our results suggest potential uses in the study the mechanism of AGR2 as a secreted prote of the tumor.In a broader sense,they also has important theoretical and practical significance for early diagnosis and prognosis of cancer.; 福建省自然科学基金项目(2012J01155); 中央高校基本科研业务费项目; 厦门大学药学院院长基金项目 |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/123535]  |
| 专题 | 药学院－已发表论文 |
| 推荐引用方式 GB/T 7714 | 刘文杰,魏龙刚,陶进,等. 癌症标记蛋白AGR2的原核表达及纯化, Prokaryotic Expression and Purification of Cancer Maker Protein AGR2[J],2015. |
| APA | 刘文杰,魏龙刚,陶进,&吕忠显.(2015).癌症标记蛋白AGR2的原核表达及纯化.. |
| MLA | 刘文杰,et al."癌症标记蛋白AGR2的原核表达及纯化".(2015). |
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