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铬胁迫对拟穴青蟹体内磷酸酶活性的影响; The Effects of Chromium Stress on the Activities of ACP and AKP in Different Tissues and Organs of Mud Crab Scylla paramamosain
蒋云霞 ; 徐华 ; 艾春香
2010
关键词拟穴青蟹 铬胁迫 磷酸酶 Scylla paramamosain chromium(Cr6+) stress phosphatase
英文摘要采用生态毒理学方法,研究了水体中Cr6+(0.5,1.0,2.0,4.0,8.0 Mg/l)胁迫1,3,5,7,9 d后对拟穴青蟹鳃、肌肉和肝胰腺中磷酸酶活性的影响,以未添加Cr6+的自然海水组为对照组.结果表明,Cr6+胁迫1 d后拟穴青蟹鳃中酸性磷酸酶(ACP)活性显著升高(P<0.05),肝胰腺、肌肉中ACP活性升高不显著(P>0.05),Cr6+胁迫9 d后,2.0,4.0,8.0Mg/l Cr6+浓度组拟穴青蟹肝胰腺中ACP活性显著降低(P<0.05).5 d后不同Cr6+浓度组拟穴青蟹鳃中碱性磷酸酶(AkP)活性一直显著升高(P<0.05),胁迫9 d后,0.5,1.0,2.0 Mg/l Cr6+浓度组与对照组差异不显著(P>0.05),4.0,8.0 Mg/l Cr6+浓度组鳃中AkP活性仍显著高于对照组(P<0.05).2.0,4.0,8.0 Mg/l Cr6+浓度组肝胰腺、肌肉中AkP活性在Cr6+胁迫1 d后显著升高(P<0.05).Cr6+胁迫显著影响拟穴青蟹生理生化效应.; A eco-toxicological experiment was conducted to investigate the effects of different concentrations of water-borne Cr6+(0.5,1.0,2.0,4.0,8.0 mg/L)stress and nature seawater(don′t contain Cr6+,as the control) on the activities of ACP and AKP in gill,hepatopancreas and muscle of mud crab Scylla paramamosain.The present results indicated that the ACP activity in gill of S.paramamosain was increased significantly after 1 d Cr6+ exposure(p<0.05),wherease,the ACP activity in hepatopancreas and muscle did not show significant changes(p>0.05).The ACP activity in hepatopancreas was significantly decreased(p<0.05) after 9 d Cr6+ exposure at the concentrations of 2.0,4.0 and 8.0 mg/L,respectively.The AKP activity in gill was significantly increased(p<0.05) after 5 d Cr6+ exposure,and it did not show significant changes(p>0.05) after 9 d Cr6+ exposure at the concentrations of 5,1.0 and 2.0 mg/L,respectively.On the other hand,the AKP activity in hepatopancreas and muscle was significantly increased(p<0.05) after 1 d Cr6+ exposure at the concentrations of 2.0,4.0 and 8.0 mg/L,respectively.Thus,chromium stress can significantly affect the physiological-biochemical parameter of Scylla paramamosian.; 国家863计划项目(2007AA091406);公益性行业(农业)科研专项(nyhyzx07-043)
语种zh_CN
内容类型期刊论文
源URL[http://dspace.xmu.edu.cn/handle/2288/102192]  
专题公共卫生-已发表论文
推荐引用方式
GB/T 7714
蒋云霞,徐华,艾春香. 铬胁迫对拟穴青蟹体内磷酸酶活性的影响, The Effects of Chromium Stress on the Activities of ACP and AKP in Different Tissues and Organs of Mud Crab Scylla paramamosain[J],2010.
APA 蒋云霞,徐华,&艾春香.(2010).铬胁迫对拟穴青蟹体内磷酸酶活性的影响..
MLA 蒋云霞,et al."铬胁迫对拟穴青蟹体内磷酸酶活性的影响".(2010).
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