Visualization of Protease Activity In Vivo Using an Activatable Photo-Acoustic Imaging Probe Based on CuS Nanoparticles

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	Visualization of Protease Activity In Vivo Using an Activatable Photo-Acoustic Imaging Probe Based on CuS Nanoparticles
	Yang, Kai ; Zhu, Lei ; Nie, Liming ; Sun, Xiaolian ; Cheng, Liang ; Wu, Chenxi ; Niu, Gang ; Chen, Xiaoyuan ; Liu, Zhuang ; Chen XY(陈小元)
刊名	http://dx.doi.org/10.7150/thno.7217
	2014
关键词	PHOTOTHERMAL ABLATION THERAPY LIVING MICE MATRIX METALLOPROTEINASES CARBON NANOTUBES AGENTS SENSITIVITY TOMOGRAPHY GRAPHENE
英文摘要	National "973" Program of China [2011CB911002, 2012CB932601, 2013CB733802, 2014CB744503]; National Natural Science Foundation of China [51002100, 51072126, 51373144, 81371596]; Intramural Research Program (IRP) of the National Institute of Biomedical Imaging and Bioengineering (NIBIB), NCI), National Institutes of Health (NIH); China scholarship Council (CSC); Herein, we for the first time report a novel activatable photoacoustic (PA) imaging nano-probe for in vivo detection of cancer-related matrix metalloproteinases (MMPs). A black hole quencher 3 (BHQ3) which absorbs red light is conjugated to near-infrared (NIR)-absorbing copper sulfide (CuS) nanoparticles via a MMP-cleavable peptide linker. The obtained CuS-peptide-BHQ3 (CPQ) nano-probe exhibits two distinctive absorption peaks at 630 nm and 930 nm. Inside the tumor microenviorment where MMPs present, the MMP-sensitive peptide would be cleaved, releasing BHQ3 from the CuS nanoparticles, the former of which as a small molecule is then rapidly cleared out from the tumor, whereas the latter of which as large nanoparticles would retain inside the tumor for a much longer period of time. As the result, the PA signal at 680 nm which is contributed by BHQ3 would be quickly diminished while that at 930 nm would be largely retained. The PA signal ratio of 680 nm /930 nm could thus serve as an in vivo indicator of MMPs activity inside the tumor. Our work presents a novel strategy of in vivo sensing of MMPs based on PA imaging, which should offer remarkably improved detection depth compared with traditional optical imaging techniques.
语种	英语
出版者	IVYSPRING INT PUBL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87598]
专题	公共卫生－已发表论文
推荐引用方式 GB/T 7714	Yang, Kai,Zhu, Lei,Nie, Liming,et al. Visualization of Protease Activity In Vivo Using an Activatable Photo-Acoustic Imaging Probe Based on CuS Nanoparticles[J]. http://dx.doi.org/10.7150/thno.7217,2014.
APA	Yang, Kai.,Zhu, Lei.,Nie, Liming.,Sun, Xiaolian.,Cheng, Liang.,...&陈小元.(2014).Visualization of Protease Activity In Vivo Using an Activatable Photo-Acoustic Imaging Probe Based on CuS Nanoparticles.http://dx.doi.org/10.7150/thno.7217.
MLA	Yang, Kai,et al."Visualization of Protease Activity In Vivo Using an Activatable Photo-Acoustic Imaging Probe Based on CuS Nanoparticles".http://dx.doi.org/10.7150/thno.7217 (2014).