Association of EGFR Mutation or ALK Rearrangement With Expression of DNA Repair and Synthesis Genes in Never-Smoker Women With Pulmonary Adenocarcinoma

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	Association of EGFR Mutation or ALK Rearrangement With Expression of DNA Repair and Synthesis Genes in Never-Smoker Women With Pulmonary Adenocarcinoma
	Ren, Shengxiang ; Chen, Xiaoxia ; Kuang, Peng ; Zheng, Limou ; Su, Chunxia ; Li, Jiayu ; Li, Bing ; Wang, Yongshen ; Liu, Lu ; Hu, Qiong ; Zhang, Jie ; Tang, Liang ; Li, Xuefei ; Zhou, Caicun ; Schmid-Bindert, Gerald ; Zheng LM(郑立谋)
刊名	http://dx.doi.org/10.1002/cncr.27603
	2012-11-15
关键词	CELL-LUNG-CANCER GROWTH-FACTOR RECEPTOR CISPLATIN PLUS GEMCITABINE RANDOMIZED PHASE-III ACTIVATING MUTATIONS MESSENGER-RNA CHEMOTHERAPY GEFITINIB ERCC1 MULTICENTER
英文摘要	Amoy Diagnostics Company Ltd., Xiamen, China; National Science Foundation of China [81172108]; Science and Technology Commission of Shanghai Municipality [06DZ19502]; BACKGROUND: Epidermal growth factor receptor (EGFR) mutation and anaplastic lymphoma kinase (ALK) rearrangement may predict the outcome of targeted drug therapy and also are associated with the efficacy of chemotherapy in patients with nonsmall cell lung cancer (NSCLC). The authors of this report investigated the relation of EGFR mutation or ALK rearrangement status and the expression of DNA repair or synthesis genes, including excision repair cross-complementing 1 (ERCC1), ribonucleotide reductase subunit M1 (RRM1), thymidylate synthetase (TS), and breast cancer-early onset (BRCA1), as a potential explanation for these observations. METHODS: In total, 104 resected lung adenocarcinomas from women who were nonsmokers were analyzed concurrently for EGFR mutations, ALK rearrangements, and mRNA expression of the ERCC1, RRM1, TS, and BRCA1 genes. EGFR mutations were detected with a proprietary detection kit, ALK rearrangements were detected by polymerase chain reaction analysis, and genetic mRNA expression was detected by real-time polymerase chain reaction analysis. RESULTS: Of 104 patients, 73 (70.2%) had EGFR mutations, and 10 (9.6%) had ALK rearrangements. ERCC1 mRNA levels in patients who had EGFR mutations were 3.44 +/- 1.94 x 10(-3), which were significantly lower than the levels in patients who were positive for ALK rearrangements and in patients who were negative for both biomarkers (4.60 +/- 1.95 x 10(-3) and 4.95 +/- 2.33 x 10(-3), respectively; P = .010). However, TS mRNA levels were significantly lower in patients who had EGFR mutations (1.15 +/- 1.38 x 10(-3) vs 2.69 +/- 3.97 x 10(-3); P = .006) or ALK rearrangements (1.21 +/- 0.78 x 10(-3) vs 2.69 +/- 3.97 x 10(-3); P = .020) than in patients who were negative for both biomarkers. CONCLUSIONS: NSCLC specimens that harbored activating EGFR mutations were more likely to express low ERCC1 and TS mRNA levels, whereas patients with NSCLC who had ALK rearrangement were more likely to express low TS mRNA levels. Cancer 2012. (C) 2012 American Cancer Society.
语种	英语
出版者	CANCER-AM CANCER SOC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90913]
专题	生物医学－已发表论文
推荐引用方式 GB/T 7714	Ren, Shengxiang,Chen, Xiaoxia,Kuang, Peng,et al. Association of EGFR Mutation or ALK Rearrangement With Expression of DNA Repair and Synthesis Genes in Never-Smoker Women With Pulmonary Adenocarcinoma[J]. http://dx.doi.org/10.1002/cncr.27603,2012.
APA	Ren, Shengxiang.,Chen, Xiaoxia.,Kuang, Peng.,Zheng, Limou.,Su, Chunxia.,...&郑立谋.(2012).Association of EGFR Mutation or ALK Rearrangement With Expression of DNA Repair and Synthesis Genes in Never-Smoker Women With Pulmonary Adenocarcinoma.http://dx.doi.org/10.1002/cncr.27603.
MLA	Ren, Shengxiang,et al."Association of EGFR Mutation or ALK Rearrangement With Expression of DNA Repair and Synthesis Genes in Never-Smoker Women With Pulmonary Adenocarcinoma".http://dx.doi.org/10.1002/cncr.27603 (2012).