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	An improved protocol for efficient one-step PCR site-directed mutagenesis with partial-overlapping primers
	Zhou, Nan ; Wu, Shanwen ; Wang, Yu ; Tong, Xinzhu ; Chen, Ruichuan ; Liu, Runzhong ; Wang Y(王瑜) ; Chen RC(陈瑞川) ; Liu RZ(刘润忠)
	2011
英文摘要	Conference Name:2011 IEEE International Symposium on IT in Medicine and Education, ITME 2011. Conference Address: Guangzhou, China. Time:December 9, 2011 - December 11, 2011.; IEEE Sapporo Section; Lanzhou University (LZU); Henan University of Technology (HAUT); Wuhan University of Technology (WHUT); East China Normal University (ECNU); Described here is an improved protocol of the reported method for one-step PCR site-directed mutagenesis with partial-overlapping primers [1]. The improvements include the new design of partial-overlapping primers, and optimized PCR amplification at the annealing temperature of 60C, instead of recommended 52C. Under such conditions, the non-specific PCR products could be remarkably reduced or even completely eliminated. Sufficient amount of specific PCR products were obtained, which allowed not only to omit the gel purification step from the reported protocol and making the procedures automated, but also to markedly reduce the cost of mutagenesis experiments. ? 2011 IEEE.
语种	英语
出处	http://dx.doi.org/10.1109/ITiME.2011.6132186
出版者	IEEE Computer Society
内容类型	其他
源URL	[http://dspace.xmu.edu.cn/handle/2288/87122]
专题	医学院－会议论文
推荐引用方式 GB/T 7714	Zhou, Nan,Wu, Shanwen,Wang, Yu,et al. An improved protocol for efficient one-step PCR site-directed mutagenesis with partial-overlapping primers. 2011-01-01.

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