Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application

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	Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application
	Jiang, Yixiang ; Fang, Lin ; Shi, Xiaolu ; Zhang, Hailong ; Li, Yinghui ; Lin, Yiman ; Qiu, Yaqun ; Chen, Qingliang ; Li, Hui ; Zhou, Li ; Hu, Qinghua ; Zhou L(周莉)
刊名	http://dx.doi.org/10.1128/JCM.00245-14
	2014
关键词	RT-PCR GENETIC-CHARACTERIZATION VIRAL GASTROENTERITIS ROTAVIRUS INFECTION HUMAN CALICIVIRUSES STOOL SPECIMENS NOROVIRUS ASTROVIRUS CHINA ADENOVIRUS
英文摘要	National Natural Science Foundation of China [81071433]; Shenzhen Public Service Platform of Pathogenic Microorganisms Repository; We developed a highly sensitive reverse transcription and multiplex real-time PCR (rtPCR) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups I and II; sapovirus genogroups I, II, IV, and V; human rotavirus A; adenovirus serotypes 40 and 41; and human astrovirus. In comparison to monoplex rtPCR assays, the sensitivities and specificities of the multiplex rtPCR ranged from 75% to 100% and from 99% to 100%, respectively, evaluated on 812 clinical stool specimens.
语种	英语
出版者	AMER SOC MICROBIOLOGY
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93645]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Jiang, Yixiang,Fang, Lin,Shi, Xiaolu,et al. Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application[J]. http://dx.doi.org/10.1128/JCM.00245-14,2014.
APA	Jiang, Yixiang.,Fang, Lin.,Shi, Xiaolu.,Zhang, Hailong.,Li, Yinghui.,...&周莉.(2014).Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application.http://dx.doi.org/10.1128/JCM.00245-14.
MLA	Jiang, Yixiang,et al."Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application".http://dx.doi.org/10.1128/JCM.00245-14 (2014).